This study aimed to develop curcumin (CMN)-loaded chitosan nanoparticles (C-NPs) as a potential drug delivery system. Unique to this research was the use of a top-down method that allows for precise control over nanoparticle size, which is critical for enhancing stability, efficacy, bioavailability, and controlled drug delivery. The study employed advanced characterization techniques, including Fourier-transform infrared spectroscopy (FTIR), to confirm the successful formation of C-NPs, along with an evaluation of drug loading capacity (LC) and percentage drug entrapment (PDE). Typically, in vitro drug release studies were conducted to evaluate the sustained release behavior of C-NPs. The results demonstrated the successful formation of C-NPs with desirable characteristics, with a high LC (86.81% ± 5.44%) and PDE (68.12% ± 4.24%), indicating efficient encapsulation of CMN, which was significantly higher compared to many existing formulations. The sustained release mechanism not only improves bioavailability and efficacy but also facilitates targeted and combination therapies, achieving a release rate of 88.24% ± 6.89% over 24 hours. Moreover, a short-term stability study conducted has proved that the prepared C-NPs might be stable up to 15 days, highlighting their practical applicability. Overall, this study provides valuable insights into the innovative development and characterization of C-NPs as a promising carrier for CMN delivery, offering significant advancements in drug delivery systems that could lead to improved therapeutic strategies.
Magdy EA, Elhassan GO, Farhana SA, Khan RA, Abdoun SA, Nachiya RAMJ, Mohamed JMM. Studies on synthesis and physiochemical evaluation of curcumin-loaded chitosan nanoparticles. J Appl Pharm Sci. 2025. Online First. http://doi.org/10.7324/JAPS.2025.220400
1. Altammar KA. A review on nanoparticles: characteristics, synthesis, applications, and challenges. Front Microbiol. 2023;14:1155622. https://doi.org/10.3389/fmicb.2023.1155622 | |
2. Mubeen B, Ansar AN, Rasool R, Ullah I, Imam SS, Alshehri S, et al. Nanotechnology as a novel approach in combating microbes providing an alternative to antibiotics. Antibiotics (Basel). 2021;10(12):1473. https://doi.org/10.3390/antibiotics10121473 | |
3. Fathi-karkan S, Zeeshan M, Qindeel M, Malekshah RE, Rahdar A, Ferreira LF. NPs loaded with zoledronic acid as an advanced tool for cancer therapy. J Drug Deliv Technol. 2023;87:104805. https://doi.org/10.1016/j.jddst.2023.104805 | |
4. Prasad S, Aggarwal BB. Turmeric, the golden spice: from traditional medicine to modern medicine. In: Benzie IFF, Wachtel-Galor S, editors. Herbal medicine: biomolecular and clinical aspects. 2nd ed. Boca Raton, FL: CRC Press/Taylor & Francis; 2011. https://doi.org/10.1201/b10787-14 | |
5. Pistritto G, Trisciuoglio D, Ceci C, Garufi A, D'Orazi G. Apoptosis as anticancer mechanism: function and dysfunction of its modulators and targeted therapeutic strategies. Aging (Albany NY). 2016;8(4):603-19. https://doi.org/10.18632/aging.100934 | |
6. Uroševi? M, Nikoli? L, Gaji? I, Nikoli? V, Dini? A, Miljkovi? V. Curcumin: biological activities and modern pharmaceutical forms. Antibiotics (Basel). 2022;11(2):135. https://doi.org/10.3390/antibiotics11020135 | |
7. Yuan S, Carter P, Bruzelius M, Vithayathil M, Kar S, Mason AM, et al. Effects of tumour necrosis factor on cardiovascular disease and cancer: a two-sample Mendelian randomization study. EBioMedicine. 2020;59:102956. https://doi.org/10.1016/j.ebiom.2020.102956 | |
8. Mohamed JM, Ahmad F, Kishore N, Al-Subaie AM. Soluble 1: 1 stoichiometry curcumin binary complex for potential apoptosis in human colorectal adenocarcinoma cells (SW480 and Caco-2 cells). Res J Pharm Tech. 2021;14(1):129-35. https://doi.org/10.5958/0974-360X.2021.00023.8 | |
9. Shahid N, Erum A, Zaman M, Tulain UR, Shoaib QU, Malik NS, et al. Synthesis and evaluation of chitosan based controlled release nanoparticles for the delivery of ticagrelor. Des Monomers Polym. 2022;25(1):55-63. https://doi.org/10.1080/15685551.2022.2054117 | |
10. Mikušová V, Mikuš P. Advances in chitosan-based nanoparticles for drug delivery. Int J Mol Sci. 2021;22(17):9652. https://doi.org/10.3390/ijms22179652 | |
11. Desai N, Rana D, Salave S, Gupta R, Patel P, Karunakaran B, et al. Chitosan: a potential biopolymer in drug delivery and biomedical applications. Pharmaceutics. 2023;15(4):1313. https://doi.org/10.3390/pharmaceutics15041313 | |
12. Yee Kuen C, Masarudin MJ. Chitosan nanoparticle-based system: a new insight into the promising controlled release system for lung cancer treatment. Molecules. 2022;27(2):473. https://doi.org/10.3390/molecules27020473 | |
13. Hong L, Li W, Li Y, Yin S. Nanoparticle-based drug delivery systems targeting cancer cell surfaces. RSC Adv. 2023;13(31):21365-82. https://doi.org/10.1039/D3RA02969G | |
14. Karthikeyan A, Senthil N, Min T. Nanocurcumin: a promising candidate for therapeutic applications. Front Pharmacol. 2020;11:487. https://doi.org/10.3389/fphar.2020.00487 | |
15. Moballegh Nasery M, Abadi B, Poormoghadam D, Zarrabi A, Keyhanvar P, Khanbabaei H, et al. Curcumin delivery mediated by bio-based nanoparticles: a review. Molecules. 2020;25(3):689. https://doi.org/10.3390/molecules25030689 | |
16. Wong C, Ho EA. Development of modular polymeric nanoparticles for drug delivery using amine reactive chemistry. J Pharm Pharm Sci. 2024;27:13148. https://doi.org/10.3389/jpps.2024.13148 | |
17. Mohamed JM, Alqahtani A, Ahmad F, Krishnaraju V, Kalpana K. Stoichiometrically governed curcumin solid dispersion and its cytotoxic evaluation on colorectal adenocarcinoma cells. Drug Des Deliv Therap. 2020;14:4639-4658. https://doi.org/10.2147/DDDT.S273322 | |
18. Ahmad MZ, Rizwanullah M, Ahmad J, Alasmary MY, Akhter MH, Abdel-Wahab BA, et al. Progress in nanomedicine-based drug delivery in designing of chitosan nanoparticles for cancer therapy. Int J Polym Mater Polym Biomater. 2021;71:602-23. https://doi.org/10.1080/00914037.2020.1869737 | |
19. Hanafy AF, Abdalla AM, Guda TK, Gabr KE, Royall PG, Alqurshi A. Ocular anti-inflammatory activity of prednisolone acetate loaded chitosan-deoxycholate self-assembled nanoparticles. Int J Nanomedicine. 2019;14:3679-89. https://doi.org/10.2147/IJN.S195892 | |
20. Prichard L, Barwick V. Preparation of calibration curves a guide to best practice. 2003. doi: https://doi.org/10.13140/RG.2.2.36338.76488 | |
21. Mohamed JMM, Alqahtani A, Kumar TVA, Fatease AA, Alqahtani T, Krishnaraju V, et al. Superfast synthesis of stabilized silver nanoparticles using aqueous allium sativum (garlic) extract and isoniazid hydrazide conjugates: molecular docking and in-vitro characterizations. Molecules. 2022;27(1):110. https://doi.org/10.3390/molecules27010110 | |
22. Mohamed JMM, Ahmad F, Alqahtani A, Alqahtani T, Krishnaraju V, Anusuya M. Studies on preparation and evaluation of soluble 1:1 stoichiometric curcumin complex for colorectal cancer treatment. Trends Sci. 2021;18(24):1403. https://doi.org/10.48048/tis.2021.1403 | |
23. Kakkar V, Singh S, Singla D, Kaur IP. Exploring solid lipid nanoparticles to enhance the oral bioavailability of curcumin. Mol Nutr Food Res. 2011;55:495-503. https://doi.org/10.1002/mnfr.201000310 | |
24. Seelan TV, Kumari HLJ, Kishore N, Selvamani P, Lalhlenmawia H, Thanzami K, et al. Exploitation of novel gum Prunus cerasoides as mucoadhesive beads for a controlled release drug delivery. Int J Biol Macromol. 2016;85:667-673, 156. https://doi.org/10.1016/j.ijbiomac.2016.01.007 | |
25. Dai F, Zhuang Q, Huang G, Deng H, Zhang X. Infrared spectrum characteristics and quantification of OH groups in coal. ACS Omega. 2023;8(19):17064-76. https://doi.org/10.1021/acsomega.3c01336 | |
26. Kumbhar S, Khairate R, Bhatia M, Choudhari P, Gaikwad V. Evaluation of curcumin-loaded chitosan nanoparticles for wound healing activity. ADMET DMPK. 2023;11(4):601-613. https://doi.org/10.5599/admet.1897 | |
27. Amin MK, Boateng JS. Enhancing stability and mucoadhesive properties of chitosan nanoparticles by surface modification with sodium alginate and polyethylene glycol for potential oral mucosa vaccine delivery. Mar Drugs. 2022;20(3):156. https://doi.org/10.3390/md20030156 | |
28. Mohamed JM, Alqahtani A, Ahmad F, Krishnaraju V, Kalpana K. Pectin co-functionalized dual layered solid lipid nanoparticle made by soluble curcumin for the targeted potential treatment of colorectal cancer. Carbohydr Polym. 2020;252:117180. https://doi.org/10.1016/j.carbpol.2020.117180 | |
29. Mohamed JM, Alqahtani A, Ahmad F, Krishnaraju V, Kalpana K. Pectin co-functionalized dual layered solid lipid nanoparticle made by soluble curcumin for the targeted potential treatment of colorectal cancer. Carbohydr Polym. 2020;252:117180. https://doi.org/10.1016/j.carbpol.2020.117180 | |
30. Mohammed MA, Syeda JTM, Wasan KM, Wasan EK. An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics. 2017;9(4):53. https://doi.org/10.3390/pharmaceutics9040053 | |
31. Ojsteršek T, Vre?cer F, Hudovornik G. Comparative fitting of mathematical models to carvedilol release profiles obtained from hypromellose matrix tablets. Pharmaceutics 2024;16:498. https://doi.org/10.3390/pharmaceutics16040498 | |
32. Visan AI, Popescu-Pelin G, Socol G. Degradation Behavior of polymers used as coating materials for drug delivery-a basic review. Polymers (Basel). 2021;13(8):1272. https://doi.org/10.3390/polym13081272 | |
33. Unagolla JM, Jayasuriya AC. Drug transport mechanisms and in vitro release kinetics of vancomycin encapsulated chitosan-alginate polyelectrolyte microparticles as a controlled drug delivery system. Eur J Pharm Sci. 2018;114:199-209. https://doi.org/10.1016/j.ejps.2017.12.012 | |
34. Fathi-Karkan S, Heidarzadeh M, Narmi MT, Mardi N, Amini H, Saghati S, et al. Exosome-loaded microneedle patches: promising factor delivery route. Int J Biol Macromol. 2023;243:125232. https://doi.org/10.1016/j.ijbiomac.2023.125232 | |
35. Zhokh A, Strizhak P. Crossover between Fickian and non-Fickian diffusion in a system with hierarchy. Microporous Mesoporous Mater. 2019;282:22-8. https://doi.org/10.1016/j.micromeso.2019.03.016 | |
36. Singh G, Pai RS. Optimization (central composite design) and validation of HPLC method for investigation of emtricitabine loaded poly(lactic-co-glycolic acid) nanoparticles: in vitro drug release and in vivo pharmacokinetic studies. Sci World J. 2014;583090 https://doi.org/10.1155/2014/583090 | |
37. Walbi IA, Ahmad MZ, Ahmad J, Algahtani MS, Alali AS, et al. Development of a curcumin-loaded lecithin/chitosan nanoparticle utilizing a box-behnken design of experiment: formulation design and influence of process parameters. Polymers (Basel). 2022;14(18):3758. https://doi.org/10.3390/polym14183758 | |
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