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Volume: 8, Issue: 10, October, 2018
DOI: 10.7324/JAPS.2018.81013



Research Article

Molecular identification, L-lactic acid production, and antibacterial activity of Bacillus strains isolated from soils

Vasana Tolieng1, Auttaporn Booncharoen2, Ratthanatda Nuhwa2, Nuttha Thongchul1, Somboon Tanasupawat2

  Author Affiliations


Abstract

Fourteen spore-forming bacteria isolated from soils were evaluated for their taxonomic characterization, lactic acid production, and antimicrobial activity. They were belonged to the genus Bacillus and were closely related to Bacillus coagulans LMG 6326T with 97.48%–98.48% similarity, based on 16S rRNA gene analyses. Repetitive genomic element-PCR (REP-PCR) fingerprinting using the primers sets; BOX-PCR, ERIC-PCR, GTG5-PCR, and REP-PCR were used to differentiate among the species. Clustering of the isolates with the PCR fingerprint dendrograms obtained two groups. Group 1 consists of two isolates, JC-3 and JC-11 (14.29%), and the rest of isolates (85.71%) were distributed in another group. Based on 16S rRNA sequence analysis and REP-PCR and the phenotypic characteristics, they were classified as a novel Bacillus species. These isolates were screened for lactic acid production and antimicrobial efficiency, and the results revealed that they produced L-lactic acid in the range of 1.7 ± 0.1 g/l–32.6 ± 0.7 g/l at 98.58% ± 0.06%–100.00% ± 0.00% optical purity. Among them, only JC-19 was found to show inhibitory activity against Kocuria rhizophila ATCC 9341.

Keywords:

Antibacterial activity, Bacillus, lactic acid, Rep- PCR, soil.



Citation: Tolieng V, Booncharoen A, Nuhwa R, Thongchul N, Tanasupawat S. Molecular identification, L-lactic acid production, and antibacterial activity of Bacillus strains isolated from soils. J App Pharm Sci, 2018; 8(10): 098-105.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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