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Volume: 3, Issue: 6, June, 2013
DOI: 10.7324/JAPS.2013.3603



Research Article

Chemical composition, antimicrobial and cytotoxic activities of Piper hispidum Sw. essential oil collected in Venezuela


Antonio Moralesa, Janne Rojasa, Laila M. Moujirb, Liliana Araujob and María Rondónc

  Author Affiliations


Abstract

This article provides an evaluation of the chemical composition, antimicrobial and cytotoxic activities of the essential oil of Piper hispidum Sw collected in the Venezuelan Andes. The chemical composition was examined by GC/MS analysis. Thirty four compounds were identified representing 95.2 % of the total oil. The major components were α-pinene (15.3 %), β-pinene (14.8 %), β-elemene (8.1 %), caryophyllene oxide (7.8 %) and δ-3-carene (6.9 %). Antimicrobial activity was observed against Gram positive bacterial strains S. aureus ATCC 6538, S. epidermides CECT232, S. saprophyticus CECT 235, B. cereus CECT496, B. subtilis CECT39 and E. faecalis CECT735 showing MIC values between 6.25 to 15 μg/mL and MBC values of 12.5 to 15 μg/mL however a low activity was observed against Gram negative bacterial strains E. coli CECT99, P. mirabilis CECT 170 and P.aeruginosa AK 958 performing MIC and MBC values of > 200 μg/mL. On the other hand, Candida albicans yeast presented a moderate activity with MIC 100-200 µg/mL and MBC > 200 μg/mL.Cytotoxic activity was also determined against HeLa (cervix carcinoma), A-459 (lung carcinoma), MCF-7 (breast adenocarcinoma) human cancer cell lines and against normal Vero cells (African green monkey kidney), exhibiting potent antiproliferative effects with IC50 values ranging from 18.6 to 37.7 µg/mL.

Keywords:

Piper hispidum, essential oil, antimicrobial activity, cytotoxic activity.



Citation: Antonio Morales, Janne Rojas, Laila M. Moujir, Liliana Araujo and María Rondón., Chemical composition, antimicrobial and cytotoxic activities of Piper hispidum Sw. essential oil collected in Venezuela. J App Pharm Sci, 2013; 3 (06): 016-020.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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