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Volume: 2 Issue: 9, September, 2012
DOI: 10.7324/JAPS.2012.2915



Research Article

Isolation and screening of extracellular proteases produced by new Isolated Bacillus sp


Hanan S. Alnahdi

  Author Affiliations


Abstract

Bacterial are well known for their ability to excrete enzymes into the environment. Bacillus sp. being industrially important organisms produces a wide variety of extra-cellular enzymes including proteases. Bacillus sp. isolated from local marine samples collected from Saudi Arabia for produced protease enzyme. The aim of this work was to evaluate protease production by different bacterial isolated from marine. Six bacterial isolates were screened for protease production. Two protease producing isolates, No.2 and No.3 were selected on the basis of gelatin hydrolyses. When the extracellular protease were examined using fermentation production medium, the date recorded the highest extracellular protease enzyme reached to (243 U/ml) by isolate No. 2. Finally the selected isolate was identify according to the morphological characteristics and named Bacillus sp No.2 EHN.

Keywords:

Protease, extracellular, enzyme, screening, isolations. Identification, gelatin ager, Bacillus. Sp



Citation: Hanan S. Alnahdi. Isolation and screening of extracellular proteases produced by new Isolated Bacillus sp. J App Pharm Sci. 2012; 2(9): 071-074.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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