Research Article | Volume: 15, Issue: 12, December, 2025
This study assesses the effect of anti-snake venom (ASV), methanolic extract of Andrographis paniculata (MAP), and ASV+MAP (50% reduced dose of ASV+MAP) in mitigating cardiotoxicity in rats. Thirty female Wistar rats were divided into five groups (n = 6): normal control, venom control (VC), and three test groups treated with ASV or MAP or ASV+MAP. Electrocardiogram was recorded at baseline, 30, 90 minutes, 24 hours, and days 7 and 14. Serum creatine kinase (CKMB) levels were measured at baseline, 24 hours, and days 7 and 14. On day 15, animals were sacrificed for histopathological analysis. Bradycardia occurred at 90 minutes in all groups except the ASV+MAP group. The amplitude of the P wave improved maximally with ASV+MAP. CKMB, which was high in the VC group, was reduced by ASV to the extent of 20% and ASV+MAP by 30%. On day 14, a decrease in CKMB was observed in all groups, lowest being the ASV+MAP group. Histopathological changes observed in the VC group were attenuated by ASV+MAP. The multipronged approach of administering MAP along with a 50% reduced dose of ASV was shown to be the best strategy for mitigating cardiotoxicity of Naja naja venom.
Prabhu PS, Roche M, Aithal PA, Pai KSR, Singh VK, Nayak AG. Mitigating cardiotoxicity of Naja naja venom: Evaluation of a multipronged strategy using anti-snake venom and methanolic extract of Andrographis paniculata. J Appl Pharm Sci. 2025;15(12):266-275. http://doi.org/10.7324/JAPS.2025.266425
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Year
Month
Neutralization of Naja naja and Daboia russelii snake venoms by aqueous plant extracts
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