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Volume: 9, Issue: 8, August, 2019
DOI: 10.7324/JAPS.2019.90809

Research Article

In vitro cytotoxic activity assay of bacteria extract derived marine sponge Haliclona fascigera toward Hela, WiDr, T47D, and Vero cell line

Neny Sandrawati1, Regina Pariatno2, Netty Suharti2, Dian Handayani2

  Author Affiliations


Symbiotic association between marine sponge and microorganism was a promising chance in the discovery of lead compound of anticancer. This association was probably concluded that symbiotic microorganism would contain the same secondary metabolites with the host. In this continuation research, we had isolated symbiotic bacteria from a marine sponge and tested for cytotoxic activity. Twenty-six isolates of bacteria derived from marine sponge Haliclona fascigera were isolated from Setan Island, West Sumatra, Indonesia. Screening of cytotoxic activity by BSLT method and MTT assay was conducted toward 21 ethyl acetate extracts of symbiotic bacteria with weight >50 mg. One bacterial extract with code H2N was very toxic according to BSLT test, while 18 isolates were toxic with LC50 ranging from 31.17 to 283.38 ppm. All of the bacterial extracts did not show a good percentage of viability (>50%) against Hela, WiDr, T47D, dan Vero cell line in MTT assay. However, bacterial extract with code H2N have shown potential cytotoxic compared to other extracts. As per the phytochemical study, this extract probably contained terpenoid group. Based on biochemical examination this bacterium, H2N, was identified as Bacillus sp.3.


Bacillus sp., cytotoxic activity, Haliclona fascigera, marine-derived bacteria, marine sponge.

Citation: Sandrawati N, Pariatno R, Suharti N, Handayani D. In vitro cytotoxic activity assay of bacteria extract derived marine sponge Haliclona fascigera toward Hela, WiDr, T47D, and Vero cell line. J Appl Pharm Sci, 2019; 9(08):066–070.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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