Home >Current Issue
Volume: 9, Issue: 8, August, 2019
In vitro cytotoxic activity assay of bacteria extract derived marine sponge Haliclona fascigera toward Hela, WiDr, T47D, and Vero cell lineAuthor Affiliations
Symbiotic association between marine sponge and microorganism was a promising chance in the discovery of lead compound of anticancer. This association was probably concluded that symbiotic microorganism would contain the same secondary metabolites with the host. In this continuation research, we had isolated symbiotic bacteria from a marine sponge and tested for cytotoxic activity. Twenty-six isolates of bacteria derived from marine sponge Haliclona fascigera were isolated from Setan Island, West Sumatra, Indonesia. Screening of cytotoxic activity by BSLT method and MTT assay was conducted toward 21 ethyl acetate extracts of symbiotic bacteria with weight >50 mg. One bacterial extract with code H2N was very toxic according to BSLT test, while 18 isolates were toxic with LC50 ranging from 31.17 to 283.38 ppm. All of the bacterial extracts did not show a good percentage of viability (>50%) against Hela, WiDr, T47D, dan Vero cell line in MTT assay. However, bacterial extract with code H2N have shown potential cytotoxic compared to other extracts. As per the phytochemical study, this extract probably contained terpenoid group. Based on biochemical examination this bacterium, H2N, was identified as Bacillus sp.3.
Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Aboul-Ela HM, Mohamed AS, Nihad MA, Galila AY, Rob WMS. Isolation, cytotoxic activity and phylogenetic analysis of Bacillus sp. bacteria associated with the red sea sponge Amphimedon ochracea. Adv Biosci Biotechnol, 2012; 3:815-23. https://doi.org/10.4236/abb.2012.37101
Bruyn Md, Stelbrink B, Morley RJ, Hall R, Carvalho GR, Cannon CH. Borneo and Indochina are major evolutionary hotspots for Southeast Asian biodiversity. Syst Biol, 2014; 63(6):879 901. https://doi.org/10.1093/sysbio/syu047
Capuccino GJ, Sherman N. Microbiology (a laboratory manual). The Benyamin/Cummings Publishing Company, Inc., New York, NY, 2001.
Case CL, Johnson TD. Laboratory experiments in microbiology. The Benyamin / Cummings Publishing Company,Inc., New York, NY, 1984.
Fotakis G, Timbrell JA. In vitro cytotoxicity assays: comparison of LDH, neu-tral red. MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett, 2006; 160:171-7. https://doi.org/10.1016/j.toxlet.2005.07.001
Handayani, Ahdinur RF, Rustini R. Antimicrobial activity of endophytic fungi from marine sponge Haliclona fascigera. J App Pharm Sci, 2015b; 5(10):154-6. https://doi.org/10.7324/JAPS.2015.501027
Handayani D, Murniati M, Rustini R. In vitro inhibitory activity of ethyl acetate extract of symbiotic bacteria isolated from the marine sponge Haliclona fascigera against Multidrug-Resistant Organism (MDRO). J App Pharm Sci, 2016b; 6(11):218-22. https://doi.org/10.7324/JAPS.2016.601134
Handayani D, Ornando R, Rustini. Antimicrobial activity screening of symbiotic fungi from marine sponge Petrosia nigrans collected from South Coast of West Sumatera, Indonesia. Int J Pharmacogn Phytochem Res, 2016a; 8(4):623-5.
Handayani D, Rasyid W, Rustini, Zainudin EN, Hertiani T. Cytotoxic activity screening of fungal extracts derived from the West Sumatran marine sponge Haliclona fascigera to several human cell line: Hela, WiDr, T47D, and Vero. J App Pharm Sci, 2018; 8(01):055-8.
Handayani D, Sandrawaty S, Murniati M, Regina R. Screening of endophytic bacteria isolated from marine sponge Haliclona fascigera for inhibition against clinical isolates of Methicillin-Resistant Staphylococcus aureus (MRSA). J App Pharm Sci, 2015a; 5(9):139-42. https://doi.org/10.7324/JAPS.2015.50926
Hoffmann F, Larsen O, Thiel V, Rapp HT, Pape T, Michaelis W, Reitner J. An anaerobic world in sponges. J. Geomicrobiol, 2005; 22:1-10. https://doi.org/10.1080/01490450590922505
Lohman DJ, Bruyn MD, Page T, Rintelen KV, Hall R, Ng PKL, Shih HTS, Carvalho GR, Rintelen TV. Biogeography of the Indo-Australian Archipelago. Annu Rev Ecol Evol Syst, 2011; 42(1):205 26. https://doi.org/10.1146/annurev-ecolsys-102710-145001
Mayer BN, Ferrign RN, Putnam JE, Nicholas DE, McLaughlin JL. Brineshrimp: a convenient general bioassay for active plants contituents. Planta Med, 1982; 45:31-4. https://doi.org/10.1055/s-2007-971236
Mayers, P Espinosa, Parr, CS Jones, Hammond GS, Dewey TA. The animal diversity web, 2014. [ONLINE] Available via http:// animaldiversity.org (Accessed 07 January 2014).
Mearns-Spragg A, Bregu KG, Boyd, JG Burgess. Cross-species introduction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Letter Appl Microbiol, 1998; 27:142-6. https://doi.org/10.1046/j.1472-765X.1998.00416.x
Moffat JG, Rudolph J, Bailey D. Phenotypic screening in cancer drug discovery-past, present, and future. Nat Rev Drug Discov, 2014; 13:588-602. https://doi.org/10.1038/nrd4366
Strobel G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiol and Mol biol Rev, 2003; 67:491-502. https://doi.org/10.1128/MMBR.67.4.491-502.2003
Taylor MW, Radax R, Steger D, Wagner M. Sponge-associated microorganism: evolution, ecology, and biotechnological potential. Microbio Mol Bio Rev, 2007; 2:295-347. https://doi.org/10.1128/MMBR.00040-06
Thomas TRA, Kavlekar DP, LokaBharathi PA. Marine drugs from sponge-microbe association-a review. Mar Drugs, 2010; 8(4):141-6. https://doi.org/10.3390/md8041417
Webster NS, Taylor M. Marine sponges, and their microbial symbionts: love and other relationships. Environ Microbiol, 2012; 14(2):335-46. https://doi.org/10.1111/j.1462-2920.2011.02460.x
WHO. World Health Statistic. WHO Press, World Health Organization, Geneva, Switerland, 2007.
Widmer N, Bardin C, Chatelut E, Paci A, Beijnen J, Levêque D. Review of therapeutic drug monitoring of anticancer drugs part two- targeted therapies. Eur J Cancer, 2014; 50:2020-36. https://doi.org/10.1016/j.ejca.2014.04.015
Xu J, Wenwei M. Overview of research and development for anticancer drugs. J Cancer Ther, 2016; 7:762-72. https://doi.org/10.4236/jct.2016.710077
Zhang HL, Hua HM, Pei YH, Yao S. Three new cyclic acyl peptides from marine Bacillus sp. Chem Pharm Bull, 2004; 52:1029-30. https://doi.org/10.1248/cpb.52.1029