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Volume: 9, Issue: 7, July, 2019
Validation and application of reversed-phase high-performance liquid chromatography for quantitative analysis of acid orange 7 and Sudan II in blusher productsAuthor Affiliations
The objective of this study was to develop and to validate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of acid orange 7 (AO7) and Sudan II (SII) in blusher products. Separation of AO7 and SII was performed using C18 column (Thermo Synergy Gold 250 mm × 4.6 mm i.d., 5 μm) with mobile phase consisting of acetonitrile-water as a solvent (1:1 v/v) and delivered in gradient manner. The mobile phase was delivered at flow rate of 0.9 ml/minute, using column temperature of 40°C. RP-HPLC was validated by assessing several performance characteristics which included selectivity, linearity and range, sensitivity, precision, accuracy, and robustness. The developed RP-HPLC was selective for the separation of AO7 and SII. Over concentration of 10.326– 41.304 μg/ml (AO7) and 9.967–39.869 (SII), the method was linear with coefficient of correlation (r) >0.999. The method was sensitive as indicated by low limit of detection and limit of quantification. The relative standard deviation (RSD) values during intra-assay and inter-assay were lower than those required by RSD Horwitz. The percentage of recovery was within acceptable ranges as required by Association of Official Analytical Chemists method. The validated RP-HPLC method has been successfully applied for the analysis of AO7 and SII in commercial blusher products, and both AO7 and SII were not detected in the tested samples.
Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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