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Volume: 9, Issue: 7, July, 2019
DOI: 10.7324/JAPS.2019.90713

Research Article

Comparison of the larvicidal and adult mosquito attractant efficacy between straw mushroom Volvariella volvacea extract and octenol (1-octen-3-ol) on mosquito vectors (Diptera: Culicidae)

Tanawat Chaiphongpachara1, Chitchanok Chitsawaeng2, Kitthisak Khlaeo Chansukh3

  Author Affiliations


Mosquito-borne diseases are a major public health issue, especially in tropical and subtropical regions. In this study, we compared the efficacy of Volvariella volvacea (Bull. ex Fr.) mushroom, an edible, commercially available mushroom species grown in Thailand with octenol (1-octen-3-ol) as a standard substance to kill larvae and attract adult, including Aedes aegypti Linnaeus and Culex sitiens Wiedemann, mosquitoes. Five concentrations of extract, including 120, 12, 1.2, 0.12, and 0.012 mg/l were selected to test the effectiveness of killing mosquito larvae. Three concentrations of extract, including 100, 10, and 1 mg/l were selected to test the effectiveness of adult female mosquito attraction. The result of the comparison of the larvicidal efficacy between V. volvacea extract and octenol in each concentration, showed that there were differences in number of dead larvae in almost all the concentrations in testing with octenol (p < 0.05), while in the adult mosquito attraction bioassay, V. volvacea extract attracted the highest number of adult female A. aegypti and C. sitiens at the 10 mg/l concentration followed by 1 and 100 mg/l, as well as the effect of octenol. The statistical comparison found that the efficiency of attracting A. aegypti and C. sitiens mosquitoes in all the concentrations of V. volvacea extract was different from octenol (p < 0.05).


Larvicidal, adult mosquito attractant efficacy, Volvariella volvacea, Aedes aegypti, Culex sitiens.

Citation: Chaiphongpachara T, Chitsawaeng C, Chansukh KK. Comparison of the larvicidal and adult mosquito attractant efficacy between straw mushroom Volvariella volvacea extract and octenol (1-octen-3-ol) on mosquito vectors (Diptera: Culicidae). J Appl Pharm Sci, 2019; 9(07):095–099.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Bureau of Vector Borne Disease, Ministry of Public Health, Thailand. 2016. Available via http://www.thaivbd.org/n/home (Accessed 08 July 2018).

Chaiphongpachara T, Sumruayphol S. Species diversity and distribution of mosquito vectors in coastal habitats of Samut Songkhram province, Thailand. Trop Biomed, 2017; 34:524-32.

Chaiphongpachara T, Laojun S. Effect of Pleurotus djamor (Rumph. ex Fr.) Boedijn mushroom extract on larval and adult Aedes aegypti (L.) and Culex sitiens Wiedemann (Diptera : Culicidae) Mosquitoes. J Chem Pharm Sci, 2018; 11:284-7. https://doi.org/10.30558/jchps.20181104007

Chaiphongpachara T, Bumrungsuk A, Chitsawaeng C, Sumchung K, Chansukh KK. Effectiveness of Pleurotus eryngii (king oyster mushroom) extract for killing larvae and attracting adult mosquito vectors in Samut Songkhram Province of Thailand. Biol Med, 2018a; 10:4. https://doi.org/10.4172/0974-8369.1000444

Chaiphongpachara T, Jittrabiab S, Nacapunchai D. Larvicidal and adult attractant efficiency of the edible mushroom Pleurotus pulmonarius on Aedes aegypti and Culex sitiens (Diptera, Culicidae) mosquitoes. Pak J Biotechnol, 2018b; 15:641-5.

Chaiphongpachara T, Sumchung K, Chansukh KK. Larvicidal and adult mosquito attractant activity of Auricularia auricula-judae mushroom extract on Aedes aegypti (L.) and Culex sitiens Wiedemann. J Appl Pharm Sci, 2018c; 8:21-5. https://doi.org/10.7324/JAPS.2018.8803

Chaiphongpachara T, Sumchung K, Bumrungsuk A, Chansukh KK. Larvicidal and adult mosquito vector attractant activity of Tremella fuciformis Berk mushroom extract on Aedes aegypti (L.) and Culex sitiens Wiedemann (Diptera: Culicidae). J Appl Pharm Sci, 2018d; 8:7-10. https://doi.org/10.7324/JAPS.2018.8902

Chaiphongpachara T, Padidpoo O, Chansukh KK, Sumruayphol S. Efficacies of five edible mushroom extracts as odor baits for resting boxes to attract mosquito vectors : a field study in Samut Songkhram Province , Thailand. Trop Biomed, 2018e; 35:653-63.

Cilek JE, Ikediobi CO, Hallmon CF, Johnson R, Onyeozili EN, Farah SM, Mazu T, Latinwo LM, Ayuk-Takem L, Berniers UR. Semi-field evaluation of several novel alkenol analogs of 1-octen-3-ol as attractants to adult Aedes albopictus and Culex quinquefasciatus. J Am Mosq Control Assoc, 2011; 27:256-62. https://doi.org/10.2987/10-6097.1

Geier M, Boeckh J. A new Y-tube olfactometer for mosquitoes to measure the attractiveness of host odours. Entomol. Exp. Appl, 1999; 92:9-19. https://doi.org/10.1046/j.1570-7458.1999.00519.x

Guha L, Seenivasagan T, Iqbal ST, Agrawal OP, Parashar BD. Behavioral and electrophysiological responses of Aedes albopictus to certain acids and alcohols present in human skin emanations. Parasitol Res, 2014; 113:3781-7. https://doi.org/10.1007/s00436-014-4044-0

Inamdar AA, Bennett JW. A common fungal volatile organic compound induces a nitric oxide mediated inflammatory response in Drosophila melanogaster. Sci Rep, 2014; 4:1-9. https://doi.org/10.1038/srep03833

Jirakanjanakit N, Saengtharatip S, Rongnoparut P, Duchon S, Bellec C, Yoksan S. Trend of temephos resistance in Aedes (Stegomyia) mosquitoes in Thailand during 2003-2005. Environ Entomol, 2007; 36:506-11. https://doi.org/10.1603/0046-225X(2007)36[506:TOTRIA]2.0.CO;2

Mike S. Medical entomology for students. Cambridge University Press, Liverpool, United Kingdom, 2016.

Largent DL. How to identify mushrooms to genus I: macroscopic features. Eureka Printing. Mad River Press, Eureka, CA, 1986.

Largent DL, Baroni TJ. How to identify mushrooms to genus VI: modern genera. Eureka Printing. Mad River Press, Eureka, CA, 1988.

Largent DL, Thiers HD. How to identify mushrooms to genus II: feld identifcation of genera. Eureka Printing, Eureka, CA, 1977.

Mau JL, Chyau CC, Li J. Tseng YH. Flavor compounds in straw mushrooms Volvariella volvacea harvested at different stages of maturity. J Agr Food Chem, 1997; 45:4726-9. https://doi.org/10.1021/jf9703314

Nkya TE, Akhouayri I, Kisinza W, David JP. Impact of environment on mosquito response to pyrethroid insecticides: facts, evidences and prospects. Insect Biochem Mol Biol, 2013; 43(4):407-16. https://doi.org/10.1016/j.ibmb.2012.10.006

Okumu FO, Madumla EP, John AN, Lwetoijera DW, Sumaye RD. Attracting, trapping and killing disease-transmitting mosquitoes using odor-baited stations-the Ifakara Odor-Baited Stations. Parasit Vectors, 2010; 3:12. https://doi.org/10.1186/1756-3305-3-12

Pimsamarn S, Sornpeng W, Akksilp S, Paeporn P, Limpawitthayakul M. Detection of insecticide resistance in Aedes aegypti to organophosphate and synthetic pyrethroid compounds in the north-east of Thailand. Dengue Bull, 2009; 33:194-202.

Roiz D, Roussel M, Munõz J, Ruiz S, Soriguer R, Figuerola J. Efficacy of mosquito traps for collecting potential west nile mosquito vectors in a natural mediterranean wetland. Am J Trop Med Hyg, 2012; 86:642-8. https://doi.org/10.4269/ajtmh.2012.11-0326

Takken W, Kline DL. Carbon dioxide and 1-octen-3-ol as mosquito attractants. J Am Mosq Control Assoc, 1989; 5:311-6.

Stuntz DE. How to identify mushrooms to genus IV: key to families and genera. Eureka Printing, Eureka, CA, 1977.

World Health Organization. Temephos in drinking-water: use for vector control in drinking-water sources and containers, 2009. Available via https://www.who.int/water_sanitation_health/dwq/chemicals/temephos.pdf (Accessed 08 July 2018).

World Health Organization. Vector-borne diseases, 2014. Available via http://www.who.int/kobe_centre/mediacentre/vbdfactsheet. pdf (Accessed 07 May 2018).

World Health Organization. Monitoring and managing insecticide resistance in Aedes mosquito populations Interim guidance for entomologists. WHO, Geneva, Switzerland, 2016.

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