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Volume: 9, Issue: 7, July, 2019
DOI: 10.7324/JAPS.2019.90706

Research Article

Synthesis, in silico study and in vitro anti-microbial evaluation of some new N-benzoyl-N'-[2-(4-chloro-phenoxy)-acetyl]-hydrazides analogs

Fares Hezam Al-Ostoot1 & 2, Yasser Hussein Eissa Mohammed1 & 3, Zabiulla1, Anup Nagaralli Kempaiah1, Shaukath Ara Khanum1

  Author Affiliations


In bacterial resistance duty, the prompt increase against existent anti-microbial drugs is a challenging universal health problem. Bacteria represent a highly significant threat globally to healthcare-associated infections, also responsible for the majority of hospital infections, which leads to an increase in the mortality and burden of worldwide healthcare duty. In this investigation, we reported the synthesis novel substitutes of phenoxy hydrazide analogs (6a–f) and have been screened for in vitro anti-bacterial and anti-fungal activities to determine the inhibition zone by using the paper disk agar diffusion technique and broth dilution to evaluate the minimum inhibitory concentration values. The structure–activity relationship suggest that among the series (6a–f), compounds (6e) with two chloro groups and (6f) with four fluoro groups showed good inhibition against pathogenic microbes. Furthermore, these results were also confirmed by the in silico study. Based on this studies, there are a scopes of developing compounds (6e) and (6f) into potent anti-microbial drugs in the near future.


Phenoxyhydrazide, zone of inhibition, in-silico study.

Citation: Al-Ostoot FH, Mohammed YHE, Zabiulla, Kempaiah AN, Khanum SA. Synthesis, in silico study and in vitro anti-microbial evaluation of some new N-benzoyl-N'-[2-(4- chloro-phenoxy)-acetyl]-hydrazides analogs. J Appl Pharm Sci, 2019; 9(07):042–049.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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