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Volume: 9, Issue: 7, July, 2019
DOI: 10.7324/JAPS.2019.90704

Research Article

β-Caryophyllene causes remyelination and modifies cytokines expression in C57BL/6 mice with experimental autoimmune encephalomyelitis

Harleson Lopes Mesquita1, Livia Beatriz Almeida Fontes1, Laetitia Alves Cinsa1, Ademar Alves Da Silva Filho2, Akinori Cardozo Nagato1, Beatriz Julião Vieira Aarestrup1, José Otávio do Amaral Corrêa2, Fernando Monteiro Aarestrup1

  Author Affiliations


The aim of this study is to evaluate the effect of β-Caryophyllene (BCP) on the production of IL-17, transcription factors (T-bet and GATA-3), and remyellination in C57BL/6 mice induced for experimental autoimmune encephalomyelitis (EAE), the model for studying pathogenesis and new therapies for multiple sclerosis. EAE was induced in three groups of C57BL/6, with administration of BCP in two groups (25 and 50 mg/kg/day) by gavage, after the 10th day of induction. At 9 days of treatment, mice were euthanized and CNS was removed for the analysis. The profiles of IL-17, T-bet, GATA-3, and the possible remyelination properties were investigated in the central nervous system (CNS) by immunohistochemistry and Weigert–Pal–Russel’s method, respectively. BCP group (50 mg/kg/day) showed a reduction of IL-17 in brain, cerebellum, and medulla (p < 0.05) and a decrease of T-bet (p < 0.05) in medulla and cerebellum, while GATA-3 was increased (p < 0.05) in cerebellum. In both BCP-treated groups were observed remyelination and better organization of myelin. In conclusion, BCP possesses markedly in vivo anti-inflammatory and neuroprotective activities and remyelination properties in EAE-mice.


β-Caryophyllene, multiple sclerosis, experimental autoimmune encephalomyelitis.

Citation: Mesquita HL, Fontes LBA, Cinsa LA, Da Silva Filho AA, Nagato AC, Aarestrup BJV, Corrêa JOA, Aarestrup FM. β-Caryophyllene causes remyelination and modifies cytokines expression in C57BL/6 mice with experimental autoimmune encephalomyelitis. J Appl Pharm Sci, 2019; 9(07):027–033.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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