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Volume: 9, Issue: 5, May, 2019
DOI: 10.7324/JAPS.2019.90513

Research Article

Antiproliferative activity of longan (Dimocarpus longan Lour.) leaf extracts

Ratna Puspita1, Maria Bintang1, Bambang Pontjo Priosoeryanto2

  Author Affiliations


Longan (Dimocarpus longan Lour.) belongs to Sapindaceae family. We examined the antiproliferative activity of longan leaf extracts against cancer-derived cell cell lines in vitro. The tested samples were water extract, ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction of longan leaf. Cytotoxicity test is against brine shrimps that was screened using Brine Shrimp Lethality Test. Antiproliferative activity assay on WEHI-164 cells (mouse fibrosarcoma cancer cell), THP-1 cells (human peripheral blood acute monocyte cell), and vero cells (non-cancer or normal cell) that was conducted using hemocytometer with Trypan Blue Dye exclusion. The 50% lethality concentration (LC50) value of water extract, ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 854.64, 305.81, 446.55, 1313.44, and 1621.8 μg/ml. Ethanol extract exhibited significant cytotoxic due to the lowest LC50 value. Ethanol extract was then used for further examination. The highest antiproliferative activity was achieved 44.93% by 600 μg/ml ethanol extract on WEHI-164 and 57.45% by 500 μg/ml ethanol extract on THP-1. It was significantly equal to doxorubicin antiproliferative activity. Ethanol extract dose had low effect to vero cells. This present study confirmed that the longan leaf ethanol extract possess marked antiproliferative activity on cancer-derived cell lines.


Antiproliferative, cancer cell lines, cytotoxicity, in vitro, longan leaf.

Citation: Puspita R, Bintang M, Priosoeryanto BP. Possible antiproliferative activity of longan (Dimocarpus longan Lour.) leaf extracts. J Appl Pharm Sci, 2019; 9(05):102–106.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abd ElMonaem HS, Abdel-Aziz NI, Morsy MA, Badria FA, ElSenduny F, El-Ashmawy MB, Moustafa MA. Synthesis, in vitro antiproliferative evaluation and molecular docking of new tetrazole-chalcone and tetrazole-pyrazoline hybrids. J Appl Pharm Sci, 2018; 8:075-87.

Apriyanto DR, Aoki C, Hartati S, Hanafi S, Kardono LBS, Arsianti A, Louisa M, Sudiro TM, Dewi BE, Sudarmono P. Antihepatitis C virus activity of a crude extract from longan (Dimocarpus longan Lour.) leaves. Jpn J Infect Dis, 2016; 69:213-20. https://doi.org/10.7883/yoken.JJID.2015.107

American Type Culture Collection (ATCC). WEHI 164 (ATCC® CRL1751™). [Online] 2014. Available via http://www.atcc.org (Accessed 8 November 2018).

Ayoola GA, Coker HAB, Adesegun SA, Obaweya K, Ezennia EC, Atangbayila TO. Phytochemical screening and antioxidant activities of some selected medical plants used for malaria therapy in Southwstern Nigeria. J Pharm Res, 2008; 7:1019-24. https://doi.org/10.4314/tjpr.v7i3.14686

Dahham SS, Asif M, Sandai D, Majid AMA, Harn GL. Antiproliferative and apoptotic activity of crude skin secretion from Malaysian Toad (Bufo asper) on in vitro colorectal cancer cells. J Appl Pharm Sci, 2017; 7:001-6. https://doi.org/10.7324/JAPS.2017.70101

Dashora N, Sodde V, Bhagat J, Prabhu KS, dan Lobo R. Antitumor activity of Dendrophthoe falcata against Ehrlich Ascites carcinoma In Swiss albino mice. Pharmaceut Crops, 2011; 2:1-7. https://doi.org/10.2174/2210290601102010001

Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer, 2010; 127:2893-917. https://doi.org/10.1002/ijc.25516

Rampel MJ, Tombuku JL. Pengujian fitokimia dan toksisitas ekstrak etanol jantung pisang kapok (Musa paradisiaca Linn.) dengan metode Brine Shrimp Lethality Test (BSLT). J Sainsmat, 2015; 4:136-47.

González M, Samudio I, Sequeda-Castañeda LG, Celis C. Cytotoxic and antioxidant capacity of extracts from Vaccinium meridionale Swartz (Ericaceae) in transformed leukemic cell lines. J Appl Pharm Sci, 2017; 7:024-30.

Handayani D, Rasyid W, Rustini, Zainudin EN, Hertiani T. Cytotoxic activity screening of fungal extracts derived from the west sumatran marine sponge Haliclona fascigera to several human cell lines: Hela, WiDr, T47D and Vero. J Appl Pharm Sci, 2018; 8:055-8.

Han N, Bakovic M. Biologically active triterpenoids and their cardioprotective and anti-inflammatory effects. J Bioanal Biomed, 2015; S12:1-11.

Hertzog DI, Tica OS. Molecular mechanisms underlaying the anti-cancerous action of flavonoids. Curr Health Sci J, 2012; 38:145-9.

Katyal P, Bhardwaj N, Khajuria R. Flavonoids and their therapeutic potential as anticancer agents; biosynthesis, metabolism and regulation. World J Pharm Pharm Sci, 2014; 3:2188-216.

Mohamed AAB, Badria FA, Maarouf AR, Abdel-Aziz NI, ElSenduny F, Abdel-Aziz AM, Bayomi SM. Synthesis, antitumor evaluation and molecular modeling study of novel benzimidazoles and pyrazinobenzimidazoles. J Appl Pharm Sci, 2017; 7:206-14.

National Cancer Institute (NCI). Chemotherapy and you: support for people with cancer. [Online] 2007. Available via www.cancer. gov/cancertopics/chemotherapy_andyou (Accessed 20 January 2018).

Paul T, Ramasubbu S. The antioxidant, anticancer and anticoagulant activities of Acanthus ilicifolius L. roots and Lumnitzera racemosa Willd. leaves, from southeast coast of India. J Appl Pharm Sci, 2017; 7:081-7.

Priosoeryanto BP, Tateyama S, Yamaguchi R, Uchida K. Antiproliferation and colony-forming inhibition activities of recombinant feline interferon (rFeIFN) on various cells in vitro. Can J Vet Res, 1995; 59:67-9. https://doi.org/10.1016/0034-5288(95)90017-9

Priosoeryanto BP, Huminto H, Wibawan IWT, Tiuria R, Tateyama S. Morphological characteristics of in vivo cultured cells derived from tumor in domestic animals. Hayati, 2002; 9:49-54.

Priosoeryanto BP, Sari R, Tiuria R, Darusman LK, Purwakusumah ED, Nurcholis W. In vitro antiproliferation activity of temulawak (Curcuma xanthorriza Roxb.) ethanol extract on MCA-B1 and MCM-B2 derived tumor cell lines. Hemera zoa Indones J Vet Sci Med, 2009; 1:29-35.

Salamah N, Widyasari E. Antioxidant activity of methanolic extract of Longan (Euphoria longan (L) Steud.) leaves using 2,2'diphenyl- 1-picrylhydrazyl radical scavenging method. Pharmaҫiana, 2015; 5:25-34.

Sari DI, Triyasmono L. Rendemen dan flavonoid total ekstrak etanol kulit batang bangkal (Nauclea subdita) dengan metode maserasi ultrasonikasi. J Pharmsci, 2017; 04(01):48-53. https://doi.org/10.20527/jps.v4i1.5755

Sukmarianti NW, Suaniti NM, Swantara IMD. Identifikasi dan uji aktivitas antikanker ekstrak spons Ianthella basta terhadap larva Artemia salina L. Cakra Kimia (Indones J App Chem), 2013; 1(1): 14-9.

Tannoury M, Saab A, Harb JEN, Makhlouf H, Assaf MD. In vitro cytotoxic activity of Laurencia papillosa, marine red algae from the Lebanese coast. J Appl Pharm Sci, 2017; 7:175-9.

Tettey CO, Ocloo A, Nagajyothi PCN, Lee KD. An in vitro analysis of antiproliferative and antimicrobial activities of solvent fractions of Taraxacum officinale (Dandelion) leaf. J Appl Pharm Sci, 2014; 4:041-5. https://doi.org/10.1080/10496475.2013.871382

World Health Organization (WHO). Prevalence of cancer worldwide. [Online] 2017. Available via http://www.who.int/mediacentre/ factsheets/fs297/en/ (Accessed 27 September 2017).

Zakaria FR, Susanto H, Hartoyo A. Pengaruh konsumsi jahe (Zingiber officinale roscoe) terhadap kadar malonaldehida dan vitamin E plasma pada mahasiswa di Pesantren Ulil Albab Kedung Badak. Bull Teknol Indust Pangan, 2000; XI:36-40.

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