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Volume: 9, Issue: 5, May, 2019
DOI: 10.7324/JAPS.2019.90513



Research Article

Antiproliferative activity of longan (Dimocarpus longan Lour.) leaf extracts

Ratna Puspita1, Maria Bintang1, Bambang Pontjo Priosoeryanto2

  Author Affiliations


Abstract

Longan (Dimocarpus longan Lour.) belongs to Sapindaceae family. We examined the antiproliferative activity of longan leaf extracts against cancer-derived cell cell lines in vitro. The tested samples were water extract, ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction of longan leaf. Cytotoxicity test is against brine shrimps that was screened using Brine Shrimp Lethality Test. Antiproliferative activity assay on WEHI-164 cells (mouse fibrosarcoma cancer cell), THP-1 cells (human peripheral blood acute monocyte cell), and vero cells (non-cancer or normal cell) that was conducted using hemocytometer with Trypan Blue Dye exclusion. The 50% lethality concentration (LC50) value of water extract, ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 854.64, 305.81, 446.55, 1313.44, and 1621.8 μg/ml. Ethanol extract exhibited significant cytotoxic due to the lowest LC50 value. Ethanol extract was then used for further examination. The highest antiproliferative activity was achieved 44.93% by 600 μg/ml ethanol extract on WEHI-164 and 57.45% by 500 μg/ml ethanol extract on THP-1. It was significantly equal to doxorubicin antiproliferative activity. Ethanol extract dose had low effect to vero cells. This present study confirmed that the longan leaf ethanol extract possess marked antiproliferative activity on cancer-derived cell lines.

Keywords:

Antiproliferative, cancer cell lines, cytotoxicity, in vitro, longan leaf.



Citation: Puspita R, Bintang M, Priosoeryanto BP. Possible antiproliferative activity of longan (Dimocarpus longan Lour.) leaf extracts. J Appl Pharm Sci, 2019; 9(05):102–106.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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