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Volume: 9, Issue: 5, May, 2019
DOI: 10.7324/JAPS.2019.90511

Research Article

Antibacterial and antidiarrheal activity of Simarouba amara (Aubl.) bark

Hegde Veena1, Nagaraj Navya1, Gowda K Sandesh2, Nayaka Boramuthi Thippeswamy1

  Author Affiliations


The present study was conducted to determine the nutritional elements in Simarouba amara (Aubl.) bark aqueous extract (SAAE) by inductively coupled plasma optical emission spectrometry (ICP-OES) and the in vitro antibacterial activity against pathogens enterotoxigenic Escherichia coli, Salmonella typhi, Staphylococcous aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa by agar well diffusion, minimum inhibitory, and bactericidal concentration. Then, antidiarrheal effect was studied on castor oil-induced diarrhea in mice model. Recorded Mg > Fe > Cu > Zn elements in SAAE invariably found to be effective against Gram-positive and Gram-negative pathogens. Effective concentration of bark showed the zone of inhibition against enterotoxigenic E. coli (200 mg/ml), S. typhi and S. aureus (300 mg/ml), and P. aeruginosa and K. pneumonia (100 mg/ml). The standard ratio between minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was meticulously recorded “one” against all pathogens, which confirms the bactericidal property. Results in mice model prominently showed that SAAE significantly (p < 0.05) reduced the frequency and number of diarrheal episodes, intestinal fluid accumulation, and intestinal transit time in dose-dependent manner. Inordinate delay in charcoal movement in the intestine positively confirmed the antispasmodic effect by reducing propulsive movement. Confirmed findings in this study naturally suggested that SAAE could be an effective antibacterial and antidiarrheal formulation.


MIC, MBC, castor-oil-induced diarrhea, intestinal motility, charcoal meal test, antidiarrheal index.

Citation: Hegde V, Nagaraj N, Gowda SK, Thippeswamy NB. Antibacterial and antidiarrheal activity of Simarouba amara (Aubl.) bark. J Appl Pharm Sci, 2019; 9(05):088–096.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Adeyemi OO, Akindele AJ. Antidiarrhoeal activity of the ethyl acetate extract of Baphia nitida (Papilionaceae). J Ethnopharmacol, 2008; 116:407-12. https://doi.org/10.1016/j.jep.2007.12.004

Alberts B, Johnson A, Lewis J, Morgan D, Raff M, Roberts. Molecular biology of the cell. Garland Science, New York, NY, 2002.

Almeida CE, Karnikowski MG, Foleto R, Baldisserotto B. Analysis of antidiarrhoeic effect of plants used in popular medicine. Rev Saude Publica, 1995; 29(6):428-33. https://doi.org/10.1590/S0034-89101995000600002

Aqil F, Khan SMA, Owais M, Ahmad I. Effect of certain bioactive plant extracts on clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus. J Basic Microbiol, 2005; 45(2):106-14. https://doi.org/10.1002/jobm.200410355

Arakha M, Pal S, Samantarrai D, Panigrahi TK, Mallick BC, Pramanik K, Mallick B, Jha S. Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interface. Sci Rep, 2015; 5:1-12. https://doi.org/10.1038/srep14813

Arunlakshana O, Schild HO. Some quantitative uses of drug antagonists. Br J Pharmacol Chemother, 1959; 14:48-58. https://doi.org/10.1111/j.1476-5381.1959.tb00928.x

Awad AB, Toczek J, Fink CS. Phytosterols decrease prostaglandin release in cultured P388D1/MAB macrophages. Prostaglandins Leukot Essent Fatty Acids, 2004; 70(6):511-20. https://doi.org/10.1016/j.plefa.2003.11.005

Banfi E, Scialino G, Bragadin CM. Development of a microdilution method to evaluate Mycobacterium tuberculosis drug susceptibility. J Antimicrob Chemother, 2003; 52:796-800. https://doi.org/10.1093/jac/dkg439

Bennett JV, Brodie JL, Benner EJ, Kirby WMM. Simplified, accurate method for antibiotic assay of clinical specimens. Appl Microbiol, 1996; 14(2):170-7.

Bhutta Z, Black RE, Hidayat A, Penny M. Therapeutic effects of oral zinc in acute and persistent diarrhea in children in developing countries: pooled analysis of randomized controlled trials. Am J Clin Nutr, 2000; 72:1516-22. https://doi.org/10.1093/ajcn/72.6.1516

Brijesh S, Daswani P, Tetali P, Antia N, Birdi T. Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: validating its traditional usage. BMC Complement Altern Med, 2009; 47(9):1-12. https://doi.org/10.1186/1472-6882-9-47

Brown JH, Taylor P. Muscarinic receptor agonists and antagonists. Goodman and Gilman's pharmacological basis of therapeutics. McGraw-Hill, New York, NY, 1996..

Chandra M. Antimicrobial activity of medicinal plants against human pathogenic bacteria. Int J Biotechnol Biol Res, 2013; 4(7):653-58.

Degu A, Engidawork E, Shibeshi W. Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model. BMC Complement Altern Med, 2016; 16:379. https://doi.org/10.1186/s12906-016-1357-9

Dosso K, Nguessan BB, Bidie AP, Gnangoran BN, Méité S, Nguessan D. Antidiarrhoeal activity of an ethanol extract of the stem bark of Piliostigma reticulatum (Caesalpiniaceae) in rats. Afr J Tradit Complement Altern Med, 2011; 2(9):242-9. https://doi.org/10.4314/ajtcam.v9i2.9

Elena B, Giuditta S, Carlo MB. Development of a microdilution method to evaluate Mycobacterium tuberculosis drug susceptibility. J Antimicrob Chemother, 2003; 52:796-800. https://doi.org/10.1093/jac/dkg439

Enzo PA. Phytochemicals from traditional medicinal plants used in the treatment of diarrhoea: modes of action and effects on intestinal function. Phytother Res, 2006; 20(9):717-24. https://doi.org/10.1002/ptr.1907

Favus MJ, Kathpalia SC, Coe FL. Kinetic characteristics of calcium absorption and secretion by rat colon. Am J Physiol, 1981; 240(5):G350-4. https://doi.org/10.1152/ajpgi.1981.240.5.G350

Giannattasio A, Guarino A, Vecchio AL. Management of children with prolonged diarrhea. F1000 Res, 2016; 5:1-11. https://doi.org/10.12688/f1000research.7469.1

Hamalainen M, Nieminen R, Asmawi MZ, Vuorela P, Vapaatalo H, Moilanen E. Effects of flavonoids on prostaglandin E2 production and on COX-2 and mPGES-1 expressions in activated macrophages. Planta Med, 2011; 77(13):1504-11. https://doi.org/10.1055/s-0030-1270762

Ismail S, Asad M. Immunomodulatory activity of Acacia catechu. Indian J Physiol Pharmacol, 2009; 53(1):25-33.

Ighadaro OM, Akinolye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid. Alexendria J Med, 2018; 287-293. https://doi.org/10.1016/j.ajme.2017.09.001

Kang CG, Hah DS, Kim CH, Kim YH, Euikyung Kim E, Jong- Shu Kim JS. Evaluation of antimicrobial activity of the methanol extracts from 8 traditional medicinal plants. Toxicol Res, 2011; 27(1):31-6. https://doi.org/10.5487/TR.2011.27.1.031

Khan AV, Ahmed QU, Shukla I, Khan AA. Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases. Asian Pac J Trop Biomed, 2012; 2(3):189-94. https://doi.org/10.1016/S2221-1691(12)60040-9

Khan IA, McChesney JD, Burandt CL. Qualitative and quantitative high performance liquid chromatographic analysis of quassinoids in in Simaroubaceae plants. Phytochem Anal, 1996; 7(4):192- 200. https://doi.org/10.1002/(SICI)1099-1565(199607)7:4< 192::AID-PCA303>3.0.CO;2-Z

Kiela PR, Ghishan FK. Physiology of intestinal absorption and secretion. Best Pract Res Clin Gastroenterol, 2016; 30(2):145-59. https://doi.org/10.1016/j.bpg.2016.02.007

Kirchhoff P, Geibel JB. Role of calcium and other trace elements in the gastrointestinal physiology. World J Gastroenterol, 2006; 12(20):3229-36. https://doi.org/10.3748/wjg.v12.i20.3229

Lemire JA, Harrison JA, Turner RJ. Antimicrobial activities of metals: mechanisms, molecular targets and application. Nat Rev Microbiol, 2013; 11:371-84. https://doi.org/10.1038/nrmicro3028

Maciel MA, Pinto AC, Arruda AC, Pamplona SG, Vanderlinde FA, Lapa AJ. Ethnopharmacology, phytochemistry and pharmacology: a successful combination in the study of Croton cajucara. J Ethnopharmacol, 2000; 70(1):41-55. https://doi.org/10.1016/S0378-8741(99)00159-2

Maddison J, Page S, Church D. Small animal clinical pharmacology. W.B. Saunders, London, UK, 2002.

Maranhão HML, Carlos FB, Vasconcelos CFB, Rolim LA, Neto PJR, Silva Neto JC. Hepatoprotective effect of the aqueous extract of simarouba amara (aublet) (simaroubaceae) stem bark against carbon tetrachloride (ccl4)-induced hepatic damage in rats. Mole, 2014a; 19:17735- 46. https://doi.org/10.3390/molecules191117735

Maranhão HML, Rolim LA, Caldas GFR, Silva-Neto JC, Wanderley AG. Gastroprotective effects of the aqueous extract of Simarouba amara (Aublet) (Simaroubaceae) stem bark on experimentally induced gastric ulcers in rodents. Int Res J Pharm App Sci, 2014b; 4(2):58-66. https://doi.org/10.3390/molecules191117735

Maranhão HML, Vasconcelos CFB, Rolim LA, Costa-Silva JH, Silva Neto JC, Wanderley AG. Acute and subacute toxicities of the aqueous extract of Simarouba amara Aublet stem bark. Int J Pharm Sci Res, 2014c; 5(12):5151-62.

Mbagwu HO, Adeyemi OO. Anti-diarrhoeal activity of the aqueous extract of Mezoneuron benthamianum Baill (Caesalpinaceae). J Ethnopharmacol, 2008; 116:16-20. https://doi.org/10.1016/j.jep.2007.10.037

Medina FS, Galvez J, Gonzalez M, Zarzuelo A, Barrett KE. Effects of quercetin on epithelial chloride secretion. Life Sci, 1997; 61(20):2049-55. https://doi.org/10.1016/S0024-3205(97)00863-1

Nemeth J, Oesch G, Kuster SP. Bacteriostatic versus bactericidal antibiotics for patients with serious bacterial infections: systematic review and meta-analysis, J Antimicrob Chemother, 2015; 70:382-95. https://doi.org/10.1093/jac/dku379

Ngo-Teke G, Kulate JR, Kuete V, Teponno RB, Tapondjou LA, Vllarem G. Antidiarrhoeal activity of extract and compound from Trilepisium madagascariense stem bark. Ind J Pharma, 2010; 42(3):157-63. https://doi.org/10.4103/0253-7613.66839

Polonsky J, Varon Z, Jacquemin H, Petitt GR. The isolation and structure of 13,18-dehydroglaucarubinone, a new antineoplastic quassinoid from Simarouba amara. Expernetia, 1978; 34(9):1122-3. https://doi.org/10.1007/BF01922904

Qing-hua Y, Li Y, Qing W, MaXiao-qin. Determination of major and trace elements in six herbal drugs for relieving heat and toxicity by ICP-AES with microwave digestion. J Saudi Chem Soc, 2012; 16(3):287-90. https://doi.org/10.1016/j.jscs.2011.01.014

4(7) 455-65.Satyajit S, Lutfun N. "Turmeric: the genus Curcuma" bioactivity of turmeric. CRC Press, Boca Raton, FL, pp 56-60, 2007.

Raj PY, Deepa K, Reddy MA, Reddeppa M, Rani ST. Evaluation of trace elements in some medicinal plants: Jatropha, Albizia, Azardicta indica and Datura plants. Eur J Pharm Med Res, 2017; 4

(7) 455-65.Satyajit S, Lutfun N. "Turmeric: the genus Curcuma" bioactivity of turmeric. CRC Press, Boca Raton, FL, pp 56-60, 2007.

Shao H, Bian CM. Determination of the content of trace elements and rates of transference in seven traditional Chinese medicines. Guangdong Trace Elements Sci, 2002; 9(11):51-4.

Sharma S, Lakshmi KS, Rajesh. T. Evaluation of antidiarrhoeal potentials of ethanolic extract of leaves of Holoptelea integrifolia in mice model. Int J PharmTech Res, 2009; 1(3):832-6.

Taylor L. Technical Data Report for Simarouba; Simarouba amara. Herbal Secrets of the Rainforest. Sage Press Inc, Austin, TX, vol. 2, pp 1-20, 2003.

Tripathi KD. Essentials of medical pharmacology. Jaypeeb Brothers Medicals Publishers (P) Ltd, New Delhi, India.

Tunaru S, Althoff TF, Nüsing RM, Diener M, Offermanns S. Castor oil induces laxation and uterus contraction via ricinoleic acid activating prostaglandin EP3 receptors. PNAS, 2012; 109(23):9179-84. https://doi.org/10.1073/pnas.1201627109

Tyagi P, Singh M, Kumari H, Kumari A, Mukhopadhyay K. Bactericidal activity of Curcumin I is associated with damaging of bacterial membrane, PLoS One, 2015; 10(3):1-15. https://doi.org/10.1371/journal.pone.0121313

Umer S, Tekewe A, Kebede N. Antidiarrhoeal and antimicrobial activity of Calpurnia aurea leaf extract. BMC Complement Altern Med, 2013; 13(21):1-5. https://doi.org/10.1186/1472-6882-13-21

Valle DL Jr. Cabrera EC, Puzon JJM, Rivera WL. Antimicrobial activities of methanol, ethanol and supercritical CO2 extracts of Philippine piper betle on clinical isolates of gram positive and gram negative bacteria with transferable multiple drug resistance, PLoS One, 2015; 11(1):1-14. https://doi.org/10.1371/journal.pone.0146349

Weaver AJJ, Shepard JB, Wilkinson RA, Watkins RL, Walton SK, Radke. Antibacterial activity of THAM trisphenylguanide against methicillin-resistant Staphylococcus aureus. PLoS One, 2014; 9(5):1-9. https://doi.org/10.1371/journal.pone.0097742

Wright CW, O'Neill MJ, Phillipson D, Warhurst DC. Use of microdilution to assess in vitro antiamoebic activities of bruceajavanica fruits, Simarouba amara stem, and a number of quassinoids. Antimicrob Agents Chemother, 1988; 32(11):1725-9. https://doi.org/10.1128/AAC.32.11.1725

Xie Y, Yang L. Calcium and magnesium ions are membrane-active against stationary-phase Staphylococcus aureus with high specificity. Sci Rep, 2016; 6:1-8. https://doi.org/10.1038/srep20628

Yadav AK, Tangpu V. Antidiarrheal activity of Lithocarpus dealbata and Urena lobata extracts: therapeutic implications. Pharm Biol, 2007; 45(3):223-9. https://doi.org/10.1080/13880200701213153

Zeng B, Chen Q, Ju M. Trace elements associated with the analysis of the efficacy of traditional Chinese medicine. Lishizhen Med Materia Med Res, 2001; 12(7):658-61.

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