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Volume: 9, Issue: 5, May, 2019
DOI: 10.7324/JAPS.2019.90508

Research Article

Protective effect of methanolic and flavonoid-rich leaf extracts of Synsepalum dulcificum (Danielli) on lead-acetate-induced toxicity in Wistar albino rats

Tajudeen O. Obafemi1, Amos Onasanya1, Akinwunmi Adeoye2 , John A. Falode2, David J. Daniel1, Ehizode F. Irefo1, Adeleke O. Ojo1, Adewale Fadaka1, Olakunle B. Afolabi1, Joseph O. Awe1, Benjamin O. Omiyale1

  Author Affiliations


The present study aims to evaluate the protective effect of methanolic leaf extract and flavonoid-rich leaf extract of Synsepalum dulcificum on lead-acetate-induced toxicity in Wistar albino rats. Forty-five animals were distributed into nine groups with five animals apiece. Group 1 served as the control and was given only distilled water throughout the course of the study. Group 2 served as the lead-induced group and was administered 50 mg/kg lead-acetate. Groups 3–8 were co-administered 50 mg/kg lead-acetate and various doses of the extracts. Group 9 was administered 40 mg/kg vitamin C in addition to 50 mg/kg lead-acetate. The study lasted for 14 days. Standard procedures were used to evaluate the hematological indices, serum total protein, urea, creatinine, as well as marker enzymes in liver and kidney of the animals. Malondialdehyde levels, superoxide dismutase, and glutathione-s-transferase activities were also estimated in the tissues. The results showed that the extracts, especially the high doses, significantly (p < 0.05) ameliorated the harmful effects of lead administration in the liver and kidney as well as in the hematological indices. The extract could, therefore, be considered as having protective effect on lead-induced toxicity in Wistar albino rats.


Glutathione, malondialdehyde, vitamin, flavonoid, biochemical.

Citation: Obafemi TO, Onasanya A, Adeoye A, Falode JA, Daniel DJ, Irefo EF, Ojo AO, Fadaka A, Afolabi OB, Awe JO, Omiyale BO. Protective effect of methanolic and flavonoid-rich leaf extracts of Synsepalum dulcificum (Danielli) on lead-acetateinduced toxicity in Wistar albino rats. J Appl Pharm Sci, 2019; 9(05):065–072.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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