Research Article | Volume: 8, Issue: 8, August, 2018

Demethylbelamcandaquinone B Isolated from Labisia pumila Enhanced Proliferation and Differentiation of Osteoblast Cells

Haryati Ahmad Hairi,  Jamia Azdina Jamal,  Nor Ashila Aladdin,  Khairana Husin,  Noor Suhaili Mohd Sofi,  Norazlina Mohamed,  Isa Naina Mohamed,  Ahmad Nazrun Shuid   
Haryati Ahmad Hairi1, Jamia Azdina Jamal2, Nor Ashila Aladdin2, Khairana Husin2, Noor Suhaili Mohd Sofi1, Norazlina Mohamed1, Isa Naina Mohamed1, Ahmad Nazrun Shuid1

1Department of Pharmacology, Faculty of Medicine, Preclinical Building, Universiti Kebangsaan Malaysia, Jalan Yaacob Latiff, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia.

2Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Aziz, 50300 Kuala Lumpur, Malaysia

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Published:  Aug 31, 2018

DOI: 10.7324/JAPS.2018.8802
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Abstract

Labisia pumila (LP) or more commonly known as Kacip Fatimah in Malaysia has received much attention due to its estrogenic effects, including its role in the treatment of osteoporosis. This study was designed to explore the active compound of LP that may be responsible for its anti-osteoporotic effects. Crude aqueous extract of Labisia pumila var alata (LPva) was fractionated into hexane (Hex), dichloromethane (DCM) and methanol (Met) solvents and their proliferative effects on mouse osteoblastic cell line (MC3T3-E1) were evaluated with MTS bioassay. The DCM fraction significantly promoted cell proliferation in a dose-dependent manner. Thin layer chromatography (TLC) was performed on the DCM fraction of LPva to separate the constituents and the potential active compound was identified. Further isolation was achieved by column chromatography (CC) and Sephadex LH-20 column chromatography. The bioactivity of the isolated compound was confirmed by its ability to replicate MC3T3-E1 accelerated proliferation and differentiation. Mass spectrometry and nuclear magnetic resonance (NMR) identified the active compound as demethylbelamcandaquinone B. Further studies are required to determine the potential of this active compound of LPva in treating osteoporosis.


Keyword:     Active compound Labisia pumila osteoporosis osteoblasts.

Citation:

Hairi HA, Jamal JA, Aladdin NA, Husin K, Sofi NSM, Mohamed N, Mohamed IN, Shuid AN. Demethylbelamcandaquinone B Isolated from Labisia Pumila Enhanced Proliferation and Differentiation of Osteoblast Cells. J App Pharm Sci, 2018; 8(08): 012-020.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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