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Volume: 8, Issue: 2, February, 2018
DOI: 10.7324/JAPS.2018.8221

Research Article

Diversity of Yeasts and Their Ethanol Production at high Temperature

Vasana Tolieng1, Sineenath Kunthiphun2, Ancharida Savarajara2, Somboon Tanasupawat3

  Author Affiliations


Thirty one yeasts isolated from sugarcane juice and process-sediments collected from different area of sugar factory were screened for ethanol production at 40°C. The isolates were belonged to 7 genera, namely Pichia, Ogataea, Millerozyma, Meyerozyma, Candida, Kluyveromyces and Saccharomyces based on phylogenetic analysis of D1/ D2 region of the large subunit ribosomal (LSU) RNA gene. The majority of the isolates were identified as Pichia kudriavzevii (Group IB, 14 isolates), Ogataea polymorpha (Group II, 5 isolates), Kluyveromyces maxianus (Group VI, 5 isolates), Pichia manshurica (Group IA, 3 isolates), while the minor isolates were Candida tropicalis (Group V, 1 isolate), Millerozyma farinosa (Group III, 1 isolate), Meyerozyma caribbica (Group IV, 1 isolate) and Sacharomyces cereviseae (Group VII, 1 isolate). The isolates produced ethanol ranged from 2.97 to 57.10 g/L at 40°C. Isolates G3- 9(1) and G3-3(1), identified as K. marxianus and S. cerevisiae showed high potential for ethanol production, 57.10 ± 0.23 and 49.42 ± 0.34 g/L, respectively.


Diversity, ethanol, Pichia, Kluyveromyces, Sacharomyces, thermotolerant yeasts.

Citation: Tolieng V, Kunthiphun S, Savarajara A, Tanasupawat S. Diversity of Yeasts and Their Ethanol Production at high Temperature. J App Pharm Sci, 2018; 8(02): 136-142.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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