Research Article | Volume: 7, Issue: 6, June, 2017

Gas chromatography-mass spectrometry analysis, antimicrobial, anticancer and antioxidant activities of n-hexane and methylene chloride extracts of Senna italica

Hassan M.F. Madkour,  Mosad A. Ghareeb,  Mohamed S. Abdel-Aziz,  Omar M. Khalaf,  Amal M. Saad,  Ahmed K. El-Ziaty,  Mamdouh Abdel-Mogib   
Hassan M.F. Madkour1, Mosad A. Ghareeb2, Mohamed S. Abdel-Aziz3, Omar M. Khalaf1, Amal M. Saad2, Ahmed K. El-Ziaty1, Mamdouh Abdel-Mogib4

1Chemistry Department, Faculty of Science, Ain Shams University, El-Khalifa El-Mamoun, 11566 Abbassia, Cairo, Egypt.

2Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, 12411 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt.

3Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, National Research Center, El Behoos Street 33, Dokki-Giza, Egypt.

4Chemistry Department, Faculty of Science, Mansoura University, Mansoura-35516, Mansoura, Egypt.

Open Access   

Published:  Jun 30, 2017

DOI: 10.7324/JAPS.2017.70604
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Abstract

The current study was aimed to investigate the antimicrobial, anticancer, and antioxidant activities of the n-hexane and methylene chloride extracts from Senna italica as well as the characterization of their chemical constituents via gas chromatography-mass spectrometry analysis (GC-MS). The results revealed that the n-hexane extract showed weak antimicrobial activity with inhibition zones were ranged from 3.8 to 7mm, and the methylene chloride extract showed a potent antimicrobial activity with inhibition zones were ranged from 4.2 to 18mm compared to five standard antibiotics. Also, according to the criteria of the American National Cancer Institute (USNCI), the n-hexane showed weak anticancer activity with IC50 equal to 81.6±4.1, 73.0±3.90, 66.8±3.12, and 92.8±4.82 µg/ml, while the methylene chloride exhibited a potent anticancer activity with IC50 of 16.9±1.30, 17.4±1.36, 18.3±1.59, and 14.2±1.18µg/ml, compared to 5-fluorouracil with IC50 of 7.9±0.28, 4.8±0.21, 8.3±0.35, and 5.4±0.20, all respectively for HePG-2, Hela, PC3, and MCF-7 tumor cell lines. The activity against ABTS radical was recorded with the methylene chloride (% inhibition= 86.3%), compared to ascorbic acid with 89.2%. Moreover, GC-MS analysis revealed that the major constituents of the n-hexane were n-hexadecanoic acid (30%), (Z,Z,Z)9,12,15-octadecadienoic acid (21%), vitamin E (7.32%), and for methylene chloride were 3-methyl-4-oxopentanoic acid (16.36%), (E)-stilbene (11.86%), and 2,6-di-tert-butylphenol (10.70%).


Keyword:     Senna italica antimicrobialanticancer ABTS GC-MS analysis.

Citation:

Madkour HMF, Ghareeb MA, Abdel-Aziz MS, Khalaf OM, Saad AM, El-Ziaty AK, Abdel-Mogib M. Gas chromatography-mass spectrometry analysis, antimicrobial, anticancer and antioxidant activities of n-hexane and methylene chloride extracts of Senna italica. J App Pharm Sci, 2017; 7 (06): 023-032.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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