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Volume: 6, Issue: 1, January, 2016
DOI: 10.7324/JAPS.2016.600106



Research Article

Identification and characterization of Lactobacillus bacterial genera most prevalent used to improve silage digestibility of important forage species for livestock sector


Fierro Roberto1 , 2, Chamorro Diana1, Pazmiño Julio1, Darwin Rueda1, Zúñiga Johana1, Ríos Diego1, Bangeppagari Manjunatha1, Muchakayala Ravi3, Mariadoss Selvanayagam4 , 5

  Author Affiliations


Abstract

The objective of this study is to isolate and characterize lactic acid bacteria and their effect as a microbial inoculants in silage digestibility of Lolium perenneTrifolium pratense (Rye grass - Red clover) (RG-RC), Avena sativa – Vicia sativa (Oat-Vetch) (O-V) and Corn Stover Zea mays (Corn) (C). The lactic acid bacteria (LAB) isolated from three mixtures were identified at 40 day of evolution of micro silages. Morphological, physiological, biochemical and molecular techniques were used to characterize the isolates lactic acid bacteria. The following species were identified from the mixture namely Lactobacillus buchneri, Lactobacillus plantarum, Lactobacillus brevis and Pediococcus acidilactici. 54 micro silages with each feed material were produced, 27 micro silos were inoculated with bacteria’s and the rest was used as a control. The nutritional value of protein, ether extract (EE), ash, energy, neutral detergent fiber (NDF), acid detergent fiber (ADF) at 20, 30 and 40 days of ensilage was compared. The percentage of each in vitro digestibility of treatments performed on day 40 was obtained that corresponded best A-V and RG-TR inoculated with bacteria as they were 35% and 41% more digestible than the control treatments appropriate, concluding that the inoculation of lactic acid bacteria facilitated improved digestibility of silage obtaining good nutritional quality, with optimal values. These results will enable future research on the relationship between LAB species and silage fermentation quality. Use of lactic acid bacteria is recommended as an additive to improve the nutritional quality of food animals as alternative in times of scarcity of fodder or as a supplement to improve the nutritional status of livestock herd.

Keywords:

Microsilage, Lactobacillus, Pediococcus, Nutritional parameters, In vitro digestibility.



Citation: Roberto F, Diana C, Julio P, Rueda D, Johana Z, Diego R, Manjunatha B, Ravi M, Selvanayagam M. Identification and characterization of Lactobacillus bacterial genera most prevalent used to improve silage digestibility of important forage species for livestock sector. J App Pharm Sci, 2016; 6 (01): 035-041.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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