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Volume: 5, Issue: 3, March, 2015
DOI: 10.7324/JAPS.2015.50304



Research Article

Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation

Lek Mun Leonga, Ahmad Zorin Sahalana, Liew Huat Tana, Nor Hidayah Mustafaa, Nor Fadilah Rajaba b

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Abstract

Collagen is an extracellular matrix protein with great importance in biomedical application. The search for collagen from various sources is intensified especially from marine source. This study was carried out to extract collagen from a Malaysian local fresh water fish, Clarias batrachus and characterized its biomedical potential In vitro. Collagen was extracted from C. batrachus skin using acetic acid method and identified using SDS-PAGE. MTT assay was performed to determine the effect of coated collagen on cell adhesion and proliferation of L929 skin fibroblast cells. Additionally, scratch assay was performed to examine the effect of collagen coating on fibroblast cell migration. Result showed that collagen extracted from C. batrachus was made up of collagen type I, which consists of two α chains (α-1 and α-2) and β chain. At 100 µg/cm2 density, collagen coating significantly increased fibroblast cell adhesion, proliferation and migration compared to negative control (p< 0.05). As a conclusion, collagen extracted from C. batrachus increased cell adhesion, proliferation and migration of fibroblast cells and has potential to be used as an alternative source of collagen.

Keywords:

Clarias batrachus, collagen extract, L929 cell, cell migration.



Citation: Lek Mun Leong, Ahmad Zorin Sahalan, Liew Huat Tan, Nor Hidayah Mustafa, Nor Fadilah Rajab. Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation. J App Pharm Sci, 2015; 5 (03): 019-023


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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