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Volume: 4, Issue: 7, July, 2014
DOI: 10.7324/JAPS.2014.40722



Research Article

Simple Spectrophotometric and HPTLC-Densitometric Methods for Determination of Cefdinir in Bulk Powder and Pharmaceuticals, and in Presence of its Hydrolytic Degradation Products

Omar Abdel-Aziz1, Maha Farouk1, Reham Nagi1, Laila Abdel-Fattah2

  Author Affiliations


Abstract

The present work describes development and validation of four different stability indicating methods for quantitative analysis of cefdinir (CFD) in bulk powder and pharmaceuticals, and in the presence of its acid and alkaline induced hydrolytic degradation products. The first method is based on derivative spectrophotometry. First derivative spectrophotometry was applied where CFD was determined at 313.4 nm in the presence of its alkaline degradation product and also second derivative spectrophotometry where CFD was determined at 298.2 nm in the presence of its acid degradation product. The second method is based on the first derivative of ratio spectrophotometry of CFD at 312 nm in the presence of its acid degradation product and at 310.2 nm in the presence of its alkaline degradation product. The third method is based on the mean centering of ratio spectrophotometry where the drug was determined at 288.4 and 284.8nm in laboratory prepared mixtures with its acid and alkaline degradation products, respectively. The fourth method is HPTLC-densitometry using diethylether-methanol-water-glacial acetic acid (6: 3: 1: 0.05, v/v) as a developing system. Due to simplicity, rapidity and accuracy of the proposed stability indicating methods, they are effective for quality control analysis.

Keywords:

Cefdinir; Derivative; Ratio spectra; Mean centering; HPTLC; Stability



Citation: Omar Abdel-Aziz, Maha Farouk, Reham Nagi, Laila Abdel-Fattah., Simple Spectrophotometric and HPTLC-Densitometric Methods for Determination of Cefdinir in Bulk Powder and Pharmaceuticals, and in Presence of its Hydrolytic Degradation Products. J App Pharm Sci, 2014; 4 (07): 129-136.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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