Home >Current Issue

Volume: 9, Issue: 2, February, 2019
DOI: 10.7324/JAPS.2019.90218



Short Communication

Distribution and antimicrobial activity of Thai marine actinomycetes

Wongsakorn Phongsopitanun1, 2, Khanit Suwanborirux3, Somboon Tanasupawat1

  Author Affiliations


Abstract

A total of 41 actinomycetes were isolated from marine samples collected in Thailand. On the basis of morphology, chemotaxonomy, and 16S rRNA gene sequence analysis, they were identified as Salinispora (13 isolates), Micromonospora (11 isolates), Nocardia (1 isolate), Verrucosispora (2 isolates), and Streptomyces (14 isolates). The antimicrobial activity screening revealed that two Micromonospora isolates, 12 Salinispora isolates and 10 Streptomyces isolates showed activity against Staphylococcus aureus ATCC 25923, Kocuria rhizophila ATCC 9341, Bacillus subtilis ATCC 6633, Escherichia coli NIHJ KC213, Candida albicans KF1, and Mucor racemosus IFO 4581. Based on this study, the production media and strains were the main factors that influenced the antimicrobial activity.

Keywords:

Antibacterial activity, marine actinomycetes, sediment, marine sponges.



Citation: Phongsopitanun W, Suwanborirux K, Tanasupawat S. Distribution and antimicrobial activity of Thai marine actinomycetes. J Appl Pharm Sci, 2019; 9(02):129–134.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

Abdelmohsen, U. R., Bayer, K., Hentschel, U. Diversity, abundance and natural products of marine sponge-associated actinomycetes. Nat Prod Rep, 2014; 31:381-399. https://doi.org/10.1039/C3NP70111E

Berdy J. Bioactive microbial metabolites. J Antibiot, 2005; 58:1-26. https://doi.org/10.1038/ja.2005.1

Bister B, Bischoff D, Ströbele M, Riedlinger J, Reicke A, Wolter F, Bull AT, Zähner H, Fiedler HP, Süssmuth RD. Abyssomicin C-A polycyclic antibiotic from a marine Verrucosispora strain as an inhibitor of the p-aminobenzoic acid/tetrahydrofolate biosynthesis pathway. Angew Chem Int Ed Engl, 2004; 43(19):2574-2576. https://doi.org/10.1002/anie.200353160

Goodfellow M, Williams ST, Mordarski M. 1988. Actinomycetes in Biotechnology, London: Academic Press Inc, p. 1-88.

Inahashi Y, Matsumoto A, Omura S, Takahashi Y. Streptosporangium oxazolinicum sp. nov., a novel endophytic actinomycete producing new antitrypanosomal antibiotics, spoxazomicins. J Antibiot (Tokyo), 2011; 64(4): 297-302. https://doi.org/10.1038/ja.2011.18

Jensen PR, Williams PG, Oh DC, Zeigler L, Fenical W. Species-specific secondary metabolite production in marine actinomycetes of the genus Salinispora. Appl Environ Microbiol, 2007; 73(4):1146-1152. https://doi.org/10.1128/AEM.01891-06

Kageyama A, Yazawa K, Nishimura K, Mikami Y. Nocardia testaceus sp. nov. and Nocardia senatus sp. nov., isolated from patients in Japan. Microbiol Immunol, 2004; 48: 271-276. https://doi.org/10.1111/j.1348-0421.2004.tb03523.x

Kawamoto I. 1989. Genus Micromonospora. In: Williams ST, Sharpe ME, Holt JG, eds. Bergey's Manual of Systematic Bacteriology, vol. 4, Baltimore: Williams & Wilkins 2442-2450.

Kwon HC, Kauffman CA, Jensen PR, Fenical W. Marinomycins A-D, antitumor-antibiotics of a new structure class from a marine actinomycete of the recently discovered genus "Marinispora". J Am Chem Soc, 2006; 128(5): 1622-1632. https://doi.org/10.1021/ja0558948

Lane DJ. 1991. 16S/23S rRNA sequencing. In E. Strackbrandt & M. Goodfellow (Eds.), Nucleic Acid Techniques in Bacterial Systematics (pp. 115-148). Chichester: Wiley.

Maldonado LA, Fenical W, Jensen PR, Kauffman CA, Mincer TJ, Ward AC, Bull AT, Goodfellow M. Salinispora arenicola gen. nov., sp. nov. and Salinispora tropica sp. nov., obligate marine actinomycetes belonging to the family Micromonosporaceae. Int J Syst Evol Microbiol, 2005; 55:1759-1766. https://doi.org/10.1099/ijs.0.63625-0

Mao J, Tang Q, Zhang Z, Wang W, Wei D, Huang Y, Liu Z, Shi Y, Goodfellow M. Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China. Int J Syst Evol Microbiol, 2007; 57:2578-2582. https://doi.org/10.1099/ijs.0.65027-0

Qin S, Chen H-H, Zhao G-Z, Li J, Zhu W-Y, Xu L-H, Jiang J-H, Li W-J. Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austro- yunnanensis in Xishuangbanna tropical rain forest revealed by culture-dependent and culture-independent methods. Envion Microbiol Report. 2012; 4: 522-531. https://doi.org/10.1111/j.1758-2229.2012.00357.x

Qin S1, Li J, Chen HH, Zhao GZ, Zhu WY, Jiang CL, Xu LH, Li WJ. Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol, 2009; 75(19): 6176-6186. https://doi.org/10.1128/AEM.01034-09

Rheims H, Schumann P, Rohde M, Stackebrandt E. Verrucosispora gifhornensis gen. nov., sp. nov., a newmember of the actinobacterial family Micromonosporaceae

Int J Syst Bacteriol, 1998; 48:1119-1127. https://doi.org/10.1099/00207713-48-4-1119

Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol, 1966; 16:313-340. https://doi.org/10.1099/00207713-16-3-313

Sripreechasak P, Tanasupawat S, Matsumoto A, Inahashi Y, Suwanborirux K, Takahashi Y. Identification and antimicrobial activity of actinobacteria from soils in southern Thailand. Trop Biomed, 2013; 30(1):46-55.

Sripreechasak P, Suwanborirux K, Tanasupawat T. Characterization and antimicrobial activity of Streptomyces strains from soils in southern Thailand. J App Pharm Sci, 2014; 4 (10): 024-031.

Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol, 1974; 28:226-231.

Stackebrandt E, Rainey FA, Ward-Rainey NL. Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol, 1997; 47:479491. https://doi.org/10.1099/00207713-47-2-479

Suriyachadkun C, Chunhametha S, Thawai C, Tamura T, Potacharoen W, Kirtikara K, Sanglier JJ. Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol, 2009; 59:1632-1637. https://doi.org/10.1099/ijs.0.003228-0

Supong K, Thawai C, Suwanborirux K, Choowong W, Supothina S, Pittayakhajonwut P. Antimalarial and antitubercular C-glycosylated benz[α]anthraquinones from the marine-derived Streptomyces sp. BCC45596. Phytochem Lett. 2012; 5: 651-656. https://doi.org/10.1016/j.phytol.2012.06.015

Tamaoka J. Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics, 1994; pp. 463-470. Edited by Goodfellow M. & O'Donnel AG. Chichester: Wiley.

Zhang H, Lee YK, Zhang W, Lee HK. Culturable actinobacteria from the marine sponge Hymeniacidon perleve: isolation and phylogenetic diversity by 16S rRNA gene-RFLP analysis. Antonie Van Leeuwenhoek, 2006: 90(2):159-169. https://doi.org/10.1007/s10482-006-9070-1

Article Metrics