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Volume: 9, Issue: 2, February, 2019
DOI: 10.7324/JAPS.2019.90210

Research Article

Phytochemical compounds of anise hyssop (Agastache foeniculum) and antibacterial, antioxidant, and acetylcholinesterase inhibitory properties of its essential oil

Ivan G. Ivanov1, Radka Z. Vrancheva2, Nadezhda T. Petkova1, Yulian Tumbarski3, Ivayla N. Dincheva4, Ilian K. Badjakov4

  Author Affiliations


Agastache foeniculum (Pursh) Kuntze (anise hyssop) is known as a medicinal and flavoring spice plant. The aim of this research was gas chromatography-mass spectrometry (GC-MS) analysis of anise hyssop essential oil and analysis of radical scavenging ability, antimicrobial activity, and acetylcholinesterase inhibitory activity. Phytochemical composition of anise hyssop wastes and the aqueous extract obtained after steam distillation was investigated. Eight compounds were identified in essential oils by GC-MS analysis, as the major ones were phenylpropenoids [estragol—93.45% of total ion current (TIC), eugenol—0.15% of TIC, and methyl isoeugenol—2.48% of TIC]. In the anise hyssop wastes extract, four pentacyclic triterpenes were identified (betulin—36.1 mg/g, betulinic acid—2.4 mg/g, oleanolic acid—160.0 mg/g, and ursolic acid—6.7 mg/g extract). Rosmarinic acid (50.6 mg/g extract) and flavonoids—myricetin (0.5 mg/g), luteolin (0.9 mg/g), and apigenin (0.6 mg/g) were detected by high-performance liquid chromatography with diode-array detection analysis of aqueous extract. The anise hyssop essential oil showed strong radical scavenging ability IC50—6.54 μl/ml. The results obtained from antimicrobial screening revealed that essential oil possessed inhibitory activity against Staphylococcus aureus ATCC 25923, Curtobacterium flaccumfaciens PM_YT, Listeria monocytogenes, Bacillus subtilis ATCC 6633, Salmonella sp., Escherichia coli ATCC 8739, Proteus vulgaris, Pseudomonas aeruginosa ATCC 9027, Klebsiella pneumoniae, and Candida albicans, while Enterococcus faecalis remained unaffected.


Agastache foeniculum, essential oil, pentacyclic triterpenes, antimicrobial activity, antioxidant activity, rosmarinic acid.

Citation: Ivanov IG, Vrancheva RZ, Petkova NT, Tumbarski Y, Dincheva IN, Badjakov IK. Phytochemical compounds of anise hyssop (Agastache foeniculum) and antibacterial, antioxidant, and acetylcholinesterase inhibitory properties of its essential oil. J Appl Pharm Sci, 2019; 9(02):072–078.

Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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