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Volume: 9, Issue: 2, February, 2019
DOI: 10.7324/JAPS.2019.90209



Research Article

Biological activity of Passiflora edulis f. Flavicarpa ethanolic leaves extract on human colonic adenocarcinoma cells

Valentina Ramirez1, Sandra Sulay Arango1, Maria Elena Maldonado2, Diego Uribe1, Johanny Aguillon3, Jenny Paola Quintero3, Nelsy Loango3

  Author Affiliations


Abstract

Studies of extracts and phytochemicals from Passiflora genus have shown promissory biological activity for cancer chemoprevention; thus, in this work, it was evaluated the anticancer potential of the ethanolic extract of Passiflora edulis f. Flavicarpa leaves over Caco-2 and SW480 colorectal adenocarcinoma cells. The effect of the extract on cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and its antiproliferative activity was assessed by clonogenic assays. In addition, by flow cytometry approaches, it was evaluated the apoptosisinducing effect and cell cycle regulation. In this way, it was established the half maximal inhibitory concentration (IC50) of the extract over both cell lines and also, it was observed the inhibition of cellular proliferation after 48 hours of exposure to the extract. On the other hand, we observed that the extract induced cell death-mechanisms, by the increasing of the proportion of necrotic and apoptotic cells; furthermore, the extract altered the distribution of cell cycle phases by increasing SubG1 and G2/M populations. Altogether, these results suggest that P. edulis is a potential source of phytochemical compounds with anticancer properties for the management of colorectal cancer patients.

Keywords:

Passiflora edulis, phytochemicals, anticancer potential, colorectal cancer.



Citation: Ramirez V, Arango SS, Maldonado ME, Uribe D, Aguillon J, Quintero JP, Loango N. Biological activity of Passiflora edulis f. Flavicarpa ethanolic leaves extract on human colonic adenocarcinoma cells. J Appl Pharm Sci, 2019;9(02):064–071.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

Aguillón J, Maldonado M, Loango N, Arango S, Landázuri P. Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspectivas En Nutrición Humana. 2013; 15(1): 13–25.

Aguillón J, Arango S, Uribe D, Loango N. Citotoxyc and apoptotic activity of extracts from leaves and juice of Passiflora edulis. J Liver Res Disord Ther. 2018: 4(2): 70–74. https://doi. org/10. 15406/jlrdt. 2018. 04. 00102

Bertho L, Santiago M, Coutinho S. Flow cytometry in the study of cell death. Memórias Do Instituto Oswaldo Cruz. 2000; 95(3): 429–33. https://doi. org/10. 1590/S0074-02762000000300020

Borges L, Linden R, Wang J. DNA damage-induced cell death. Cell Res. 2008; 18(1): 17–26. https://doi. org/10. 1038/cr. 2007. 110

Correa R, Peralta R, Haminiuk, Maciel G, Bracht A, Ferreira I. The past decade findings related with nutritional composition, bioactive molecules and biotechnological applications of Passiflora spp. (Passion fruit). Trends in Food Science & Technology. 2016; 58(November) 79–95. https://doi. org/10. 1016/j. tifs. 2016. 10. 006

da Silva J, Cazarin B, Colomeu C, Batista G, Meletti M, Paschoal R, de Lima R. Antioxidant activity of aqueous extract of passion fruit (Passiflora edulis) leaves: In vitro and in vivo study. Food Research International. 2013: 53(2): 882–890. https://doi. org/10. 1016/j. foodres. 2012. 12. 043

Dhawan K, Dhawan S, Sharma A. Passiflora: A review update. Journal of Ethnopharmacology. 2004; 94: 1–23. https://doi. org/10. 1016/j. jep. 2004. 02. 023

Ferreres F, Sousa C, Valentão P, Andrade B, Seabra M, Gil-Izquierdo Á. New C-deoxyhexosyl flavones and antioxidant properties of Passiflora edulis leaf extract. Journal of Agricultural and Food Chemistry. 2007; 55(25): 10187–10193. https://doi. org/10. 1021/jf072119y

Franken N, Rodermond H, Stap J, Haveman J, Van Bree C. Clonogenic assay of cells in vitro. Nature Protocols. 2006; 1(5): 2315–9. https://doi. org/10. 1038/nprot. 2006. 339

Gutiérrez Y, Martínez M, Henriques A, del Barrio G. Análisis de flavonoides en una fracción butanólica obtenida de Phyllanthus orbicularis HBK Flavonoids analysis of a butanol fraction obtained from Phyllanthus orbicularis HBK. Revista Cubana de Farmacia. 2010; 44(3): 367–373.

Hingorani R, Deng J, Elia J, McIntyre C, Mittar D. Detection of Apoptosis Using the BD Annexin V FITC Assay on the BD FACSVerseTM System. BD Biosciences. 2011 1–12.

Ingale G, Hivrale U. Pharmacological studies of Passiflora sp . and their bioactive compounds. Plant Science. 2010; 4(10): 417–426.

Li H, Zhou P, Yang Q, Shen Y, Deng J, Li L, Zhao D. Comparative studies on anxiolytic activities and flavonoid compositions of Passiflora edulis ' edulis ' and Passiflora edulis ' flavicarpa . ' Journal of Ethnopharmacology. 2011; 133: 1085–1090. https://doi. org/10. 1016/j. jep. 2010. 11. 039

Lim T. (2012). Passiflora edulis. In Springer Sciences (Ed. ), Edible Medicinal and Non- Medicianl Plants: (4): 147–165). https://doi. org/10. 1007/978-94-007-4053-2_21

Lin C, Kao C, Huang S, Li C, Chen , Li H, Chen Y. Chemical constituents of fruit chells Passiflora edulis. Chemistry of Natural Compounds. 2016; 52(2): 273–274. https://doi. org/10. 1007/s10600-016-1626-3

Montoya Y, Orozco P, Arango S, Maldonado, M, Aguillón J. Evaluation of cytotoxic activity of the aqueous extract of passion fruit ( Passiflora edulis ) on groups of cell lines. In IEEE (Ed. ), 2013 Pan American Health care Excahges (PAHCE) ( 6–10).

Neira C. 2003. The effects of yellow passion fruit, Passiflora edulis Flavicarpa, phytochemicals on cell cycle arrest and apoptosis of leukemia lymphoma MOLT-4 cell line. University of Florida.

Patel S. Morphology and pharmacology of Passiflora edulis: A review. Journal of Herbal Medicine and Toxicology. 2009; 3(1): 1–6.

Patel S, Soni H, Mishra K, Singhai A. Recent updates on the genus Passiflora : A review. Int. J. Res. Phytochem. Pharmacol. 2011; 1(1): 1–16.

Pessoa C, Veras L, Leyva A, Amaral E, Odorico M. 2006 . Anticancer potential of Northeast Brazilian plants. In M. T. H. K. and Athers (Ed. ), Lead Molecules from Natural Productus 197–211https://doi. org/10. 1016/S1572-557X(05)02012-X

Puricelli L, Dell'Aica I, Sartor L, Garbisa S, Caniato R. Preliminary evaluation of inhibition of matrix-metalloprotease MMP-2 and MMP-9 by Passiflora edulis and P. foetida aqueous extracts. Fitoterapia. 2003; 74(3): 302–304. https://doi. org/10. 1016/S0367-326X(03)00023-6

Ramírez V, Arango S, Uribe D, Maldonado M, Aguillón J. Effect of the Ethanolic Extract of Passiflora edulis F . Flavicarpa Leaves on Viability, Cytotoxicity and Apoptosis of Colon Cancer Cell Lines. J. Chem. Pharm. Res. 2017; 9(6): 135–139.

Rudnicki M, de Oliveira M, Veiga T, Reginatto F, Dal-Pizzol F, Fonseca J. Antioxidant and antiglycation properties of Passiflora alata and Passiflora edulis extracts. Food Chemistry. 2007: 100(2): 719–724. https://doi. org/10. 1016/j. foodchem. 2005. 10. 043

Schieber M, Chandel N. ROS Function in Redox Signaling and Oxidative Stress. Curr Biol. 2014; 24(10): 1–25. https://doi. org/10. 1016/j. cub. 2014. 03. 034

Stockert J, Blázquez-Castro A, Ca-ete M, Horobin R, Villanueva A. MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets. Acta Histochemica. 2012; 114(8): 785–796. https://doi. org/10. 1016/j. acthis. 2012. 01. 006

Sujana N, Ramanathan S, Vimala V, Sundaram M, Pemaiah B. Antitumour potential of Passiflora incarnate against ehrlich ascites carcinoma. Int. J. Pharm. Pharm. Sci. 2012; 4(2): 17–20.

Sznarkowska A, Kostecka A, Meller K, Bielawski K. Inhibition of cancer antioxidant defense by natural compounds. Oncotarget. 2017 8(9): 15996–16016. https://doi. org/10. 18632/oncotarget. 13723

Zas P, John S. Diabetes and Medicinal Benefits of Passiflora edulis. Int J Food Sci Nutr Diet, 2016; 5(2): 265–269.

Zeraik M, Yariwake H, Wauters J, Tits M, Angenot L. Passion fruit rinds (Passiflora edulis): isoorientin quantification by HPLC and evaluation of antioxidant (radical scavenging) capacity. Quim. Nova. 2012; 35(3): 541–545. https://doi. org/10. 1590/S0100-40422012000300019

Zucolotto S, Fagundes C, Reginatto H, Ramos F, Castellanos L, Duque C, Schenkel E. Analysis of C-glycosyl flavonoids from South American Passiflora species by HPLC-DAD and HPLC-MS. Phytochem. Analysis. 2011; 23: 232–239https://doi. org/10. 1002/pca. 1348

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