Research Article | Volume: 7, Issue: 1, January, 2017
Research Article | Volume: 7, Issue: 1, January, 2017
Catechin is a phytochemical largely present in Acacia catechu which is known for its biological potential, but its utilization is greatly limited by its low bioavailability. In the present research work catechin rich extract has been chosen for converting it into nanoformulation by emulsion solvent evaporation technique with minimized dosing employing Eudragit L 100 as a polymer for enhancing its bioavailability. Catechin entrapment efficiency of NF1, NF2 and NF3 nanoformulations were found to be 75 %, 62 % and 40 % respectively with decreased entrapment with an increased polymer concentration. SEM characterization of nanoparticles revealed the occurrence of both asymmetrical as well as aggregated particles in nano-meter range (100 nm to 400 nm).The nanoformulations were subjected for antioxidant activity by DPPH method and in vitro bioavailability by in vitro digestion method. Nano formulations displayed higher antioxidant activity (85.64 % - 92. 47 %) compared to Curcumin (80.58 %, used as standard for comparison) and pure extract (83.08 %). In vitro bioavailability of total polyphenols and Catechin was higher in acidic pH compared to alkaline pH for all the nanoformulations with a maximum bioavailability of 45 to 59 percentage respectively in an acidic media as shown by NF1.Thus, the findings proved significant enhancement in the antioxidant activity and in vitro bioavailability of catechin nanonization of active catechin rich extract using biodegradable polymer Eudragit L 100 in the form of nanocapsules.
Monika P, Basava Raj BV, Chidambara Murthy KN, Ahalya N, Gurudev K. Nanocapsules of Catechin Rich Extract for Enhanced Antioxidant Potential and In Vitro Bioavailability. J App Pharm Sci, 2017; 7 (01): 184-188.
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