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Volume: 5, Issue: 3, March, 2015
DOI: 10.7324/JAPS.2015.50307



Research Article

Prophylactic efficacy and possible mechanisms of oligosaccharides based standardized fenugreek seed extract on high-fat diet-induced insulin resistance in C57BL/6 mice

Amit D Kandhare1, Subhash L Bodhankar1, Vishwaraman Mohan2, Prasad A Thakurdesai2

  Author Affiliations


Abstract

The present work was aimed to study the efficacy and possible mechanism of oligosaccharides based standardized fenugreek seed extract (SFSE-OS) on high-fat diet (HFD)-induced insulin resistance in male C57BL/6 mice. The effects of 12 weeks of oral administration of SFSE-OS (30, 60 and 100 mg/kg, twice daily) were evaluated on HFD fed mice for anthropomorphic, glycemic, gene expression related and histopathological parameters. Separate groups of mice with vehicle co-administered with HFD and low-fat diet (LFD) were maintained as HFD control and LFD control respectively. Twelve weeks of SFSE-OS (60 and 100 mg/kg, p.o.) administration showed significant prophylactic effects on HFD induced insulin resistance in terms of body weight, plasma glucose and insulin levels, glycated hemoglobin, insulin resistance (IR), area under the curve (AUC) of plasma glucose during oral glucose tolerance and intraperitoneal insulin tolerance. Furthermore, HFD-induced mRNA expression changes in adipose tissue, liver and skeletal muscle were prevented by SFSE-OS co-administration. Histology of sections of the pancreas showed the normal architecture in all groups of mice. SFSE-OS showed promising efficacy in prevention of HFD-induced insulin resistance through modulation of Glut-2, Glut-4, IRS-2 and SREBP-1c expression.

Keywords:

Fenugreek seeds; Oligosaccharides; High-fat diet; Insulin resistance; Glut-2; Glut-4; IRS-2; SREBP-1c.



Citation: Amit D Kandhare, Subhash L Bodhankar, Vishwaraman Mohan, Prasad A Thakurdesai. Prophylactic efficacy and possible mechanisms of oligosaccharides based standardized fenugreek seed extract on high-fat diet-induced insulin resistance in C57BL/6 mice. J App Pharm Sci, 2015; 5 (03): 035-045.


Copyright: The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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