In vitro antibacterial, antioxidant, and cytotoxicity evaluations of Musa paradisiaca cv. Sekaki florets from Sarawak, Malaysia

Muhammad Mirza Ariffin1, Heng Yen Khong1*, Nyotia Nyokat1, Gee Moi Liew1, Ahmad Sazali Hamzah2, Korawinwich Boonpisuttinant3 1Faculty of Applied Sciences, Universiti Teknologi MARA, Sarawak, Malaysia. 2Centre for Synthesis and Polymer Technology, Institute of Science, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia. 3Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Thanyaburi, Thailand.


INTRODUCTION
Vegetables, fruits, and spices have been used extensively since the primitive days to cure and alleviate ailments such as diarrhea, headache, and nausea. From time to time, researchers have managed to identify, isolate, and extract compounds that exhibit therapeutic and medicinal properties from various parts of plants such as tannin, ascorbic acids, and carotenoids. The advancement in science has enabled these compounds to be synthesized in laboratories on a large scale to be utilized in the medical and pharmaceutical industries. Nowadays, the increasing concern of health promotion has increased the demand for products containing functional components. It was found that agricultural industry by-products contain several bioactive and functional compounds such as antioxidants, flavonoids, and anthocyanins (Sitthiya et al., 2018).
Generally, banana plants of different species or varieties produce edible fruits with various tastes, sizes, colors, and other characteristics. The fruits are known to be an excellent reservoir of potassium which aids in maintaining muscle functions and preventing muscle spasms. Besides that, the fruits also contain vitamins such as A, B 6 , C, and D which when consumed will help in boosting the immune system and ensuring proper metabolic functions . However, it is worth noting that the nutritional and medicinal properties of Musa spp. are not only limited to the fruits but also other parts such as blossoms, peels, roots, and seeds (Padam et al., 2014). Unfortunately, very few works have been conducted on the biological and chemical evaluations on Musa spp. blossoms as previous studies only focused on parts such as peels and pulps.
The banana blossoms, for instance, have long been used traditionally to treat diabetes and asthma as well as malnourishment especially in infants (Das et al., 2020), while the juice from the male flowers was found to provide relief for stomach problems in people of different age groups (Elancheran and Jayamuthunagai, 2014). The rich saponin content of the blossoms could be beneficial in maintaining healthy cholesterol levels and boosting the immune system (Adeolu and Enesi, 2013). A previous study also revealed that the blossom could be effective in managing noncommunicable diseases due to its potential to suppress the angiotensin-Iconverting enzyme and β-glucuronidase (Acharya et al., 2016). Another study targeted among lactating career women who consumed lactogenic biscuits formulated with banana blossom flour revealed that there was an increase in expressed breast milk among the women (Nordin et al., 2019). The authors noted that this effect could be contributed by the galactagogue property of banana blossoms which elevated breast milk production in women. Despite possessing ethnomedicinal properties, due to the lack of exposure and knowledge, the blossoms or florets are often regarded as unimportant and discarded as agricultural waste. This drawback has limited the application of the blossoms to be used only as organic fertilizers in the plantations instead of being properly utilized to their full potentials.
In recent years, several studies have been undertaken in exploring the potential of the banana by-products to serve as the source of bioactive compounds and phytochemicals. A study by Padam et al. (2014), for example, has discovered antioxidants, namely, epigallocatechin and its derivatives, from the banana flowers. Antioxidants such as epigallocatechin have been accepted to exhibit antimicrobial (Nikoo et al., 2018), anticancer (Chen et al., 2020, neuroprotective (Singh et al., 2016), and chemopreventive capabilities (Du et al., 2012, Hussain andAshafaq, 2018). It is also revealed that the Musa spp. blossoms serve as natural sources of valuable phytochemicals, especially flavonoids and terpenoids which are known to exhibit potent antioxidative properties (Mahmood et al., 2011). Although humans are equipped with built-in antioxidant mechanisms against cancer-causing free radicals damage, it seems to be beneficial to obtain extra antioxidants in the diet, especially from fruits and vegetables (Baskar et al., 2011).
Previous pharmacological studies on the blossoms of Musa spp. revealed that they possess antidiarrheal, antiulcerative, hypoglycemic, and hypocholesterolemia activities (Imam and Akter, 2011). The blossom extracts were also found to show cytotoxic and antiproliferative activities against several cancer cells such as human colon cancer cells, HT29, and HCT-116, as well as HeLa and breast cancer cells (Arun et al., 2018;Dahham et al., 2015;Nadumane and Timsina, 2014). However, despite the elaborate studies conducted to evaluate the pharmacological potentials of Musa spp. blossoms, there are still very few pieces of literature that are reported on the isolation of specific compounds responsible for the activities. Thus, more studies should be focused on targeting the compounds which could be developed into products of substantial medicinal importance. Even though synthetic materials are predominantly used in fields such as medical and pharmaceutical (Johnson, 2019;Velmurugan, 2018), there has been a growing interest in evaluating the potential of plants phytochemicals as alternatives to synthetic substances (Cao et al., 2017;Francini-Pesenti et al., 2019;Kapinova et al., 2018). Since phytochemicals are found abundantly in plant tissues (Xiao and Bai, 2019), it would be worthwhile to explore more on the presence of phytochemicals in banana blossoms which are expected to exhibit beneficial properties.
While there are many published studies on the phytochemical analysis and biological assays of various Musa spp. varieties, there are very few studies on the chemical and biological evaluation of local Musa spp. in Sarawak. Thus, this study aims to assess the phytochemical constituents as well as the pharmaceutical potential of local Musa spp., particularly the Musa paradisiaca Sekaki variety.

Plant materials
The fresh blossom of M. paradisiaca was collected from Banana Tree Sdn Bhd, Kuching, Sarawak, Malaysia. The blossom was first separated into the bracts part and the florets part. The florets (4.535 kg) were then cleaned and air-dried under adequate ventilation for 8 days at room temperature (24°C-26°C). The dried florets were ground into a fine powder using an electric cutting mill. The ground sample was later stored in a vacuum storage bag before being further processing.

Extracts preparation
The M. paradisiaca florets powder (790 g) was first extracted with hexane by the maceration method for 24 hours at room temperature. The extract was filtered through Whatman No. 1 filter paper and the filtrate was concentrated in vacuo using rotavapor (BUCHI,Switzerland) to remove the solvent as well as moisture. The extraction process was repeated using various solvents, namely, chloroform, ethyl acetate, and methanol. The weights and yields percentages (%w/w) of the crude extracts were 70.7 g (8.9%), 55.8 g (7.06%), 37.1 g (4.7%), and 41.4 g (5.24%) for hexane, chloroform, ethyl acetate, and methanol extracts, respectively. All the extracts obtained were properly sealed in an airtight container and stored at room temperature before subjected to chemical and biological evaluations.

Total phenolic content (TPC)
The Folin-Ciocalteu (FC) colorimetric assay was chosen to measure the TPC of florets extracts by using the modified protocol originally reported by Singh and Aggrawal (2018) and Johari and Khong (2019). Samples with a concentration of 1 mg/ml each were initially prepared by dissolving 5 mg of each extract in 5 ml of methanol. The FC reagent was prepared beforehand by diluting one part of the reagent with 10 parts of deionized water (1:10). About 0.75 ml of the reagent was combined and mixed with 100 µl of each extract in a glass vial and the mixture was then allowed to equilibrate for 10 minutes at ambient temperature. 0.75 ml of sodium carbonate (Na 2 CO 3 ) was added, and the mixture was shaken gently and incubated for 90 minutes at room temperature. The absorbance of the samples was then measured at 725 nm using UV-Vis spectrophotometer (Perkin Elmer, Lambda 25, US) and compared to the gallic acid standard calibration curve (0.2-1.0 mg/ml). TPC was expressed as milligrams of gallic acid equivalents per gram of the extract. The TPC values of each extract were obtained based on the equation generated from the gallic acid calibration curve (y = 0.0004x -0.0163; R 2 = 0.9996) in Figure 1.

Total flavonoid content (TFC)
The aluminum chloride colorimetric method adapted from Chandra et al. (2014) was used to determine the flavonoid content in the extracts and quercetin was used as the standard. The stock quercetin solution (1 mg/ml) was prepared by dissolving 10 mg quercetin in 10 ml methanol and different concentrations of quercetin (3.125 -25 µg/ml) were then prepared by serial dilution. The extracts (1 mg/ml) were also prepared by dissolving the extracts in methanol. 2 ml of the standards or the extracts was then mixed with 2% aluminum chloride and incubated at room temperature for 60 minutes. The absorbance of the standards and all the extracts was measured at 420 nm using a UV-Vis spectrophotometer (Perkin Elmer, Lambda 25, US). The quercetin calibration curve (y = 0.0642x + 0.0335; R 2 = 0.9996) obtained in Figure 2 was then used to calculate the TFC in each extract. The results were expressed as mg quercetin equivalents per gram of extract.

2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity
The scavenging activity of the extracts was evaluated by the spectrophotometric method adapted and modified from Pyrzynska and Pękal (2013). Ascorbic acid and absolute ethanol were used as the standard and working solution, respectively. The samples were prepared by dissolving the samples in absolute ethanol, followed by the addition of DPPH. The control was prepared by dissolving the DPPH in absolute ethanol. All the samples were vigorously shaken and incubated in the dark for at least 90 minutes at room temperature prior to analysis. The absorbance was measured in a UV spectrometer at 517 nm. The radical inhibition activity was calculated using Eq. (1). A graph of percent inhibition of DPPH radicals (%) against the concentrations of samples was plotted and the inhibitory concentration (IC 50 ) values were then calculated as follows: Absorbance of control−Absorbance of sample Absorbance of control × 100 (1)

Preparation of culture media
Nutrient agar (NA) and nutrient broth (NB) (Merck) were used as the culture media for the antimicrobial assays. Both media were prepared according to the manufacturer's directions and then sterilized at 121°C for 15 minutes in an autoclave. The media were allowed to cool at around 40°C before being used.

Preparation of inoculum
Two types of each Gram-positive (Streptococcus pyogenes and Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa and Escherichia coli) microorganisms were prepared according to the method described by Johari and Khong (2019). The NB was used as the diluent and each of the organisms was inoculated into the diluent separately, and stored overnight at 40°C.

Minimum inhibitory concentration (MIC)
This assay is generally used to determine the lowest concentration of a substance which inhibits the microscopically visible growth of microorganisms. The sample concentrations were used as the basis for the interpretation of the in vitro data. The stock solutions (1,800 µg/ml) were prepared in advance by dissolving 3.6 mg of each extract in 2 ml dimethyl sulfoxide (DMSO). The rows A and B of the 96-well plate were filled with the stock solution (100 µg/ml), while rows B-H were filled with sterile NB (100 µg/ml). The NB and sample at row B were well mixed and the mixture was shifted downward into each well from rows C to H to achieve a serial dilution by a factor of two (1,800, 900, 450, 225, 112.5, 56.25, 28.13, and 14.07 µg/ml, respectively). The inoculated bacteria (100 µg/ml) were then introduced into each well and the plate was covered with the lid, sealed, and incubated at 40°C for at least 24 hours. The base of the wells was later examined for any presence of pellets  as well as changes in turbidity which signified the growth of microbes.

Minimum bactericidal concentration (MBC)
To revalidate the results from MIC, MBC assay, which represents the concentration capable of killing up to 99% of bacteria, was carried out by observing the bacterial growth in the agar plates. Agar plates were prepared beforehand by dissolving NA powder in distilled water and the mixture was then autoclaved for 15 minutes at 121°C. The lukewarm NA was later poured aseptically into sterile Petri dishes. A micropipette was used to transfer 20 µg/ml of the content of the first clear stage in the 96well plate from the previously conducted MIC assay onto the agar plate. A sterile cotton bud was then used to gently streak the agar surface and the Petri dish was incubated for at least 24 hours at 37°C. The MBC result was interpreted by observing the growth of the bacteria on the agar plate. If no growth was observed, the MBC was interpreted to correlate with the MIC and if growth was observed, the MBC was interpreted as one step higher than the MIC instead. A substance or compound is deemed as bactericidal if both the MIC and MBC values are close to each other (Tripathi, 2013). Both the MIC and MBC of the extracts were compared to that of positive control, streptomycin sulfate, which is a commercially available as an water-soluble antibiotic.

Cytotoxicity on cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay
The anticancer activity was tested by using the method adapted from Sumathy (2018). The DU-145 human prostate cancer cell lines and HeLa (human cervical cancer) cells were cultivated first in Dulbecco's Modified Eagle Medium, enriched with antibiotics and 10% fetal bovine serum in a 96-well plate. Each of the wells was filled with 200 μl of the cell suspension, sealed, and incubated for 24 hours at 37°C in a 5% CO 2 atmosphere to promote the formation of a confluent monolayer. The cells' monolayer in each well was exposed to various concentrations of extract and nurtured for 24 and 48 hours. Next, MTT (5 mg/ml) was added and the medium was incubated for another 4 hours at 37°C. The medium was discarded, and the formazan crystal left was dissolved in 100 μl DMSO. The absorbance was measured in a microplate reader at 570 nm. The cytotoxicity was calculated using Eq. (2).

Percentage viability (%) =
Test optical density Control optical density × 100 The IC 50 values of the extract against the cell lines at 24 and 48 hours were later determined. The assay was repeated by replacing the extract with chemotherapeutic drugs doxorubicin hydrochloride, cisplatin, and fluorouracil.

Statistical analysis
All the analyses (TPC, TFC, antioxidant activity, and cytotoxicity) were carried out in triplicate to minimize the error and the results are presented as mean ± standard deviation (n = 3). The data obtained (Tables 1-3) were subjected to one-way analysis of variance and the significant differences between the means in different samples were determined by Tukey's post-hoc test at p ≤ 0.05 using Statistical Package for the Social Sciences for Windows (V.24). The correlation coefficient (R 2 ) between the TPC and antioxidant activity of the extracts was calculated using GraphPad Prism for Windows (v 8.3.0) to determine their relationship.

Total phenolic content (TPC)
The TPC results of different extracts of M. paradisiaca florets are shown in Table 1. The TPC for chloroform extract (333.03 ± 0.01 mg Gallic acid equivalent (GAE)/g extract) and ethyl acetate extract (481.53 ± 0.01 mg GAE/g extract) was higher compared to the TPC of methanol (180.90 ± 0.01 mg GAE/g extract) and hexane (165.90 ± 0.01 mg GAE/g extract).
The TPC results correlated with the study on M. paradisiaca L. (cv. Nendran) blossoms which demonstrated that the ethyl acetate fraction recorded the highest phenolic content (21.52 mg GAE/g extract) as compared with the other solvent fractions (Nisha and Mini, 2014). The TPC recorded in this study was also higher compared to that in the M. paradisiaca (cv. P. Rastali) variety from another Malaysia region with TPC of 68.65 mg GAE/g of extract (Marikkar et al., 2016).  The results obtained also portrayed that the differences in phenolic contents in M. paradisiaca florets extracts can be deduced to be dependent on the types of solvents used for extraction. Several studies have investigated the correlation of different solvents with the amount of phenolics extracted. Do et al. (2014) investigated the effect of various solvents on the phenolic contents extracted from Limnophila aromatica and found that the extract obtained by 100% ethanol showed the highest TPC. Another study by Han et al. (2016) showed that the ethyl acetate and acetone extracts of Ramaria botrytis recorded higher phenolic contents compared to the methanol and hexane extracts.
TPC assay is useful in determining the number of phenolic compounds in the plant samples. Phenolics have been the focus of many recent studies due to their antioxidative and free radical scavenging potentials (Agatonovic-Kustrin et al., 2018;Chandra et al., 2014). The ability of the phenolics to act as reducing agents by donating hydrogen and scavenging singlet oxygen in compounds gives rise to their antioxidant activity (Kruk et al., 2005;Sroka, 2005). Phenolics compounds are also found to prevent the peroxidation of lipids by inhibiting the action of several types of oxidizing enzymes such as lipoxygenases (Chandra et al., 2014;Khennouf et al., 2009). Generally, since higher phenolic content is associated with improved bioactivity, the ethyl acetate extract was expected to exhibit good antioxidant and antibacterial properties.

Total flavonoid content (TFC)
The ranges of the TFC values (Table 1) of the extracts are from 0.1625 mg QE/g extract for hexane extract to 0.5294 mg QE/g extract for methanol extract and they decrease in the following order: methanol > ethyl acetate > chloroform > hexane.
The trend observed in TFC was different compared to that in TPC, in which the TFC of the extracts increased with the increase of the polarity of the solvents used. However, the flavonoids' content obtained was relatively higher as compared to the values recorded in other M. paradisiaca flower varieties from Kuala Terengganu, Malaysia, with TFC ranging from 0.164 mg QE/g extract to 0.25 mg QE/G extract (Us et al., 2016).
Flavonoids are one of the most studied subclasses of polyphenols which can be found in a variety of plants. They are found to possess unique properties such as anti-inflammatory, antiviral, restraining pathogenic bacteria, and protecting against ultraviolet radiation damage (D' Amelia et al., 2018;Han et al., 2016;Hwang et al., 2015). The polarity of extracting solvents used is crucial as it greatly enhances the solubility of various antioxidant compounds (Do et al., 2014;Muhamad et al., 2014). Hexane was found to record the lowest TFC which may be attributed to its nonpolar nature which inhibits its ability to dissolve polar molecules such as flavonoids. Our findings were supported by previous studies on Salacia chinensis (Ngo Van et al., 2017), Isatis tinctoria (Wakeel et al., 2019), and Zia mays L. (Nawaz et al., 2019) which signified that the extraction of flavonoids was improved in solvents with higher polarity.

Antioxidant activity
The results in Figure 3 show the DPPH inhibition activity by the M. paradisiaca florets extracts and standard at varying concentrations. The IC 50 obtained for ascorbic acid was 15.33 µg/ml, while each extract recorded IC 50 values ranging between 38.26 µg/ml and 74.26 µg/ml. Among all the extracts, the maximum antioxidant potential was observed in ethyl acetate (IC 50 = 38.26 µg/ml), followed by chloroform (IC 50 = 47.73 µg/ml) NA is the IC 50 values higher than 1,000 µg/ml (no activity). The cytotoxicity levels: very active is the IC 50 values lower than 20 µg/ml, moderately active is the IC 50 values between 20 and 100 µg/ml, and poorly active is the IC 50 values between 100 and 1,000 µg/ml. -* denotes inactive selectivity index (SI). The data are expressed as mean ± SD and different superscript letters ( a-b and A-D for DU-145 i-iii and I-V for HeLa) in the column indicate denote significant differences at p < 0.05, Tukey's test. and methanol (IC 50 = 61.90 µg/ml), and it was lowest in hexane extract (IC 50 = 74.26 µg/ml). In comparison, the IC 50 of the flower bract of M. paradisiaca variety in Iraq reported by Lafta (2020) was 168.04 µg/ml, while another study by Joseph et al. (2014) on M. paradisiaca (AAB Nendran) floret extracts recorded IC 50 ranging between 63 µg/ml and 242 µg/ml. The variety of these IC 50 values signifies that the antioxidative potential of banana florets is dependent on the region of cultivation as well as the cultivars of Musa species. On the other hand, the antioxidative property in this study showed a linear correlation with the phenolic content in the extracts (R 2 = 0.8984) in which increasing phenolic content contributed to better antioxidant capacity (Fig. 4). The result correlates with a study by Pereira et al. (2015), which found that the antioxidative potential was dependent on the number of phenolic compounds present in the Byrsonima spp. plants. DPPH is a stable free radical with a strong purple intensity which is widely used for the spectrophotometric measurement of antioxidant activity in plant extracts. Upon reduction by compounds through the transfer of electron or hydrogen, it undergoes discoloration and acquires a yellowish hue.
DPPH is preferred mainly due to its ability to detect the presence of active compounds even at low concentrations and short analysis time (Do et al., 2014). The low free radical inhibition potential shown by hexane may be contributed by the lack of polyphenolic compounds present in the extract. Plants' phenolics are known to exhibit strong antioxidant activity toward harmful free radicals due to their reducing character which gives them hydrogendonating property as well as singlet oxygen scavengers (Chandra et al., 2014).

Antimicrobial activity
The antimicrobial activity of M. paradisiaca florets against all the Gram-positive (S. aureus and S. pyogenes) and Gramnegative (E. coli and P. aeruginosa) bacteria were quantitatively and qualitatively assessed from the absence or growth of bacteria in both the MIC and MBC assays. Based on the MIC results in Table 2, all the extracts displayed an active inhibitory effect toward all the tested pathogens with values ranging from 14.07 to 450 µg/ml. The extracts were highly potent against E. coli with MIC values comparable to that of streptomycin sulfate (14.07 µg/ml), followed by P. aeruginosa (28.13 µg/ml), whereas the weakest inhibition was observed in both S. aureus and S. pyogenes (450 µg/ml). The antibacterial property of the extracts was further extended to MBC assay and the data obtained correlated with the MIC assay in which the strongest inhibition was observed in E. coli, followed by P. aeruginosa, S. aureus, and S. pyogenes, respectively (E. coli > P. aeruginosa > S. aureus = S. pyogenes).
The MIC and MBC values are inversely proportional to the antibacterial property, in which lower concentrations signify stronger repressive activity. The results recorded are similar to the study reported by Jawla et al. (2012) which revealed that the M. paradisiaca flowers were potent against several pathogenic bacteria. Some of the bacteria used in this present study are commonly related to food-borne diseases, and since all the extracts showed inhibitory effect against these strains, this depicts that M. paradisiaca flowers could be utilized to suppress food-borne illnesses such as diarrhea and is a natural alternative to commercially available chemical preservatives used in food products.

Cytotoxicity activity
The cytotoxicity of the M. paradisiaca floret extracts on DU-145 cells (human prostate cancer), HeLa cells (human cervical cancer), and human dermal fibroblasts (normal cells) was investigated by MTT assay. Table 3 shows the cytotoxicity (IC 50 values) of the extracts on cancer cells, and Figure 1 shows % cell viability of normal cells. The cytotoxicity of the floret extracts on DU-145 and HeLa cells was a time-dependent inhibitory effect. At 24 hours, the cytotoxicity on DU-145 and HeLa cancer cells was found only in the MpC extract with the IC 50 values of 624.13 ± 227.44 µg/ml and 522.15 ± 37.17 µg/ml, respectively. The activity was lower than doxorubicin hydrochloride (IC 50 for DU-145 of 94.62 ± 18.03 µg/ml and IC 50 for HeLa of 90.27 ± 6.88 µg/ml) and cisplatin (IC 50 for DU-145 of 70.01 ± 3.04 µg/ml and IC 50 for HeLa of 55.28 ± 2.05 µg/ml). At 48 hours, the MpEA extract showed the highest cytotoxicity on the DU-145 cells (IC 50 value of 37.94 ± 9.28 µg/ml), which was comparable to anticancer drug 5-fluorouracil (IC 50 value of 32.50 ± 8.30 µg/ml) but lower than doxorubicin hydrochloride (IC 50 value of 0.74 ± 0.11 µg/ml)  and cisplatin (6.30 ± 0.29 µg/ml), whereas the MpC extract showed the highest cytotoxicity on HeLa cells with the IC 50 value of 444.47 ± 5.91 μg/ml, which were lower than anticancer drug including doxorubicin hydrochloride (IC 50 value of 1.87 ± 0.13 µg/ml), cisplatin (IC 50 value of 6.23 ± 0.38 µg/ml), and 5-fluorouracil (IC 50 value of 308.33 ± 19.57 µg/ml). The MpM extracts showed no cytotoxicity on both cancer cells. However, the activity of the MpEA extract on DU-145 at 48 hours was classified as moderate active (IC 50 of 20-100 µg/ml), and the other extracts were classified as inactive compounds (IC 50 > 500 µg/ml) following the criteria of active compounds from National Cancer Institute (Tanamatayarat et al., 2003).
All extracts at the concentration below 100 µg/ml exhibited no cytotoxicity on human dermal fibroblasts (a normal cell) for 24 and 48 hours. The MpEA and MpC extracts at the concentration of 1,000 µg/ml for 24 and 48 hours showed cytotoxicity on human dermal fibroblasts ( Fig. 5E and F), since they gave less than 80% cell viability when compared with the control (Buapool et al., 2013). Likewise, the anticancer drugs at low concentrations showed higher cytotoxicity on human dermal fibroblasts. In addition, the MpEA extract demonstrated the highest selectivity index (SI) of 21.10 on DU-145 cancer cell at 48 hours which was superior to cisplatin and 5-fluorouracil, whereas the MpC extract showed slightly lower SI on both cell lines.
MTT assay is generally implemented in colorimetric cytotoxicity evaluation by determining the survival rate of cells through the color change of yellow tetrazolium dye MTT to purple insoluble formazan crystal. The color change is induced by the reducing property of Nicotinamide adenine dinucleotide phosphate-dependent oxidoreductase enzyme in the active mitochondria of healthy cells. The intensity of the purple color formed is directly proportional to the enzymatic activity of the cells, in which the deeper purple color indicates a higher number of healthy living cells (Fatma Sri et al., 2015;Mosmann, 1983). The inhibitory effect demonstrated by the M. paradisiaca floret in this present study may be attributed by the presence of bioactive compounds in the extract, typically polyphenols and flavonoids which induce the apoptotic effect on cancerous cells. Bioactive compounds have long been shown to play a significant role in preventing the development of terminal illnesses such as cancer (Muniraj et al., 2019;Pan et al., 2009;Kris-Etherton et al., 2002). A previous study by Nadumane and Timsina (2014) also revealed that the ethanolic extract of M. paradisiaca flower managed to induce an antiproliferative effect on the HeLa cells with an IC 50 value of less than 10 µg/ml.

CONCLUSION
The findings from this study are evidence that M. paradisiaca cv. Sekaki florets exhibited high antioxidant and strong antimicrobial potentials as well as moderate cytotoxicity against the DU-145 human prostate cancer cell lines. Additionally, the higher phenolic and flavonoid contents are supported by the higher antioxidant property as compared to the cultivars used in the previous studies. The notable antioxidant and cytotoxicity performance depicted may be contributed by the presence of flavonoids and other phenolic constituents in the florets. However, the phytochemical constituents of the florets are dependent on the region of cultivation as well as cultivars of the Musa species. The florets which are a locally consumed functional food could be a potential antioxidant agent and could be used as a medicament against illnesses associated with free radical damages. Future studies are suggested to explore the vast medicinal values by assessing the in vivo biological activity as well as identify the phytochemical constituents to validate their pharmacological activities.