Research Article | Volume: 16, Issue: 8, August, 2026
Research Article | Volume: 16, Issue: 8, August, 2026
Dendrophthoe pentandra (L.) Miq., a hemiparasitic mistletoe widely used in Indonesian traditional medicine, is abundant in flavonoids; nevertheless, many of these compounds are inadequately characterized beyond the well-known flavonols. This study aimed to isolate and characterize a putative genistein-like compound, metabolomics standard initiative level 2–3, from the ethyl acetate fraction of leaves using a multi-instrument analytical approach. Reverse phase high performance liquid chromatography analysis revealed prominent peaks with retention times comparable to those of a genistein reference in both analytical and preparative conditions. The Fourier transform infrared spectroscopy spectrum of the isolate exhibited functional groups characteristic of an isoflavone framework, including phenolic O–H stretching, conjugated C=O carbonyl bands, and aromatic C=C and C–O vibrations. High-resolution liquid chromatography–electrospray ionization–tandem mass spectrometry quadrupole time-of-flight analysis detected a deprotonated molecular ion [M–H]- at m/z 269.0449, along with diagnostic fragment ions consistent with retro-Diels–Alder cleavage commonly observed in isoflavone aglycones. Although the analysis remained semi-quantitative and lacked orthogonal confirmation (e.g., nuclear magnetic resonance and peak-purity assessment), the combined analytical evidence was consistent with a tentative genistein-like annotation. Semi-quantitative recalculation indicated an estimated concentration of 0.5267 µg/mL in the injected ethyl acetate fraction, corresponding to an approximate relative abundance of 2.63% within the analyzed fraction. Because this value was below the estimated limit of quantification, the result should be interpreted as a relatively low-abundance estimate rather than a validated absolute quantitative measurement. These findings provide preliminary evidence supporting the possible occurrence of a genistein-like compound within the Loranthaceae family, although definitive structural confirmation requires further investigation.
Merdana IM, Lazuardi M, Susilowati S, Samirana PO, Mustofa I, Hamid IS, Yuniarti WM, Soeharsono. Isolation and Tentative Characterization of a Genistein-like Compound from the Mistletoe Dendrophthoe pentandra (L.) Miq. J Appl Pharm Sci. 2026;16(8):601–612. https://doi.org/10.1177/22313354261461003
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