Research Article | Volume: 16, Issue: 6, June, 2026

Thai Probiotic Strains Protect Against Diabetic Kidney Injury in HFD/STZ-induced Type 2 Diabetic Rats via Gut Microbiota Modulation

Chattramat Jaiyim,  Jirakit Suppamanta,  Sasikarn In-um,  Kattika Tongdee,  Marut Tangwattanachuleeporn,  Anongnard Kasorn,  Malai Taweechotipatr   

Chattramat Jaiyim1,2, Jirakit Suppamanta2, Sasikarn In-um3, Kattika Tongdee3, Marut Tangwattanachuleeporn3, Anongnard Kasorn4, Malai Taweechotipatr2,5

1Applied Medical Sciences Program, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.

2Center of Excellence in Probiotics, Srinakharinwirot University, Bangkok, Thailand.

3Department of Medical Sciences, Faculty of Allied Health Science, Burapha University, Chonburi, Thailand.

4Department of Basic Medical Science, Faculty of Medicine, Vajira Hospital, Navamindradhiraj University, Bangkok, Thailand.

5Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.

Open Access   

Published:  Apr 22, 2026

DOI: 10.1177/22313354261427942
PDF [ 6.5 MB]
Request Permission
Share
Download Citation
Print
Download PDF
Abstract

Diabetes mellitus (DM) is a metabolic disorder in which blood glucose levels remain abnormally high (fasting plasma glucose ≥126 mg/dL). Type 2 diabetes mellitus (T2DM) represents the most common form of diabetes which frequently causes kidney inflammation that results in severe complications. Probiotics, which are live beneficial microbes, have been recognized for their supportive role in human health. This work examined three Thai probiotic strains, Limosilactobacillus reuteri TF314, Lacticaseibacillus paracasei MSMC39-1, and Bifidobacterium animalis TA1, for their ability to protect the kidneys in Wistar rats fed a high-fat diet and treated with streptozotocin (STZ) to induce T2DM. Experimental animals were randomly divided into six groups: control, T2DM, TF314, MSMC39-1, TA1, and a mix group (TF314, MSMC39-1, and TA1). All the experimental rats received oral probiotic supplementation for four months. The probiotic treatments given to STZ-induced rats reduced fasting blood glucose and HbA1c levels and improved glucose tolerance. They also helped to reduce kidney injury by decreasing blood urea nitrogen (BUN) and creatinine concentrations and by suppressing pro-inflammatory markers such as TNF-α while raising IL-6. Moreover, changes in gut microbiota were observed, particularly the restoration of gut symbionts Akkermansia muciniphila and an increase in L. reuteri that may underlie better glycemic control and renal protection. The histological examination revealed that probiotic-treated rats experienced reduced kidney tissue damage. This study demonstrates that Thai probiotic strains show potential to minimize kidney problems which occur in T2DM patients.


Keyword:     Probiotics diabetes mellitus kidney inflammation high-fat diet non-communicable diseases

Citation:

Jaiyim C, Suppamanta J, In-um S, Tongdee K, Tangwattanachuleeporn M, Kasorn A, Taweechotipatr M. Thai Probiotic Strains Protect Against Diabetic Kidney Injury in HFD/STZ-induced Type 2 Diabetic Rats via Gut Microbiota Modulation. J Appl Pharm Sci. 2026;16(6):176-189. https://doi.org/10.1177/22313354261436142

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HTML Full Text

Reference

Article Metrics
43 Views 35 Downloads 78 Total

Year

Month

Related Search

By author names